Research Laboratories, Catholic University, Campobasso, Italy.
Thromb Res. 2013 Aug;132(2):288-92. doi: 10.1016/j.thromres.2013.06.022. Epub 2013 Jul 13.
Numerous epidemiological studies suggest that exposure to flavonoid-rich fruits has beneficial influence on risk factors for cardiovascular disease. We investigated whether intake of orange juice (OJ) could affect whole blood (WB) procoagulant activity.
17 healthy subjects (aged 31 ± 1.5 SEM 10 males) were randomized to receive, according to a cross-over design, either red or blond OJ, enriched or free of anthocyanins, respectively. After one week run-in period on a controlled diet, the subjects were randomly allocated to receive either type of OJ for 4 weeks, with a 4-week wash-out period. Venous blood was collected on citrate before and at the end of each treatment period. WB was incubated with or without an inflammatory stimulus (tumor necrosis factor-α or bacterial endotoxin LPS). Procoagulant activity was evaluated by a one-stage clotting assay. Tissue factor (TF) and TF pathway inhibitor (TFPI) were measured in plasma by ELISA.
Intake of either type of OJ caused a prolongation of unstimulated and stimulated WB clotting times, without any difference between the two treatments. Intake of OJ did not modify TF levels. On the contrary, an increase in circulating TFPI antigen was detected following either treatment.
Orange juice intake by healthy volunteers decreases procoagulant activity, possibly through mechanisms independent of its anthocyanin content.
许多流行病学研究表明，摄入富含类黄酮的水果对心血管疾病的危险因素有有益影响。我们研究了橙汁（OJ）的摄入是否会影响全血（WB）的促凝血活性。
17 名健康受试者（年龄 31 ± 1.5 SEM 10 名男性）按照交叉设计随机分为两组，分别摄入富含或不含花青素的红或黄橙汁。经过一周的对照饮食适应期后，受试者被随机分配接受两种 OJ 中的任意一种，为期 4 周，洗脱期为 4 周。在每个治疗期之前和结束时，采集柠檬酸盐抗凝的静脉血。WB 在存在或不存在炎症刺激物（肿瘤坏死因子-α或细菌内毒素 LPS）的情况下孵育。通过一步凝血测定法评估促凝血活性。通过 ELISA 在血浆中测量组织因子（TF）和 TF 途径抑制剂（TFPI）。
摄入任何一种 OJ 都会延长未刺激和刺激的 WB 凝血时间，两种处理之间没有差异。OJ 的摄入不会改变 TF 水平。相反，在两种处理后都检测到循环 TFPI 抗原增加。
健康志愿者饮用橙汁可降低促凝血活性，其机制可能与其花青素含量无关。
