Umoh J U, Cox J H, Schneider L G
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Zentralbl Veterinarmed B. 1990 May;37(3):222-8. doi: 10.1111/j.1439-0450.1990.tb01050.x.
Twenty isolates of street rabies virus were recovered in mouse neutroblastoma cells from 84 rabies suspect brain specimens from Nigeria dogs and a cat. They were characterized with the Tübingen monoclonal antibody panels directed against nucleocapsid and glycoprotein antigens. Antigenic variations were detected both with antinucleocapsid (anti-NC) and antiglycoprotein (anti-GP) monoclonal antibodies (mabs). One isolate reacted positively with anti-NC mab P41 which hitherto has been known to react positively with polar rabies. Another isolate did not react with anti-NC mab 187.5; a reaction normally seen with ERA/SAD strains of rabies virus. With anti-GP mabs it was possible to group the isolates by their area of origin. Isolates from Plateau State were not neutralized by anti-GP mabs ERA 543 and P44.7.2. The isolates studied had glycoprotein antigenic patterns different from the pattern for low egg passage (LEP) Flury strain vaccine virus used in Nigeria for immunization of dogs.
从尼日利亚84份犬和1份猫的狂犬病疑似脑标本中，在小鼠成神经细胞瘤细胞中分离出20株街狂犬病病毒。使用针对核衣壳和糖蛋白抗原的图宾根单克隆抗体组合对它们进行了鉴定。抗核衣壳（抗-NC）和抗糖蛋白（抗-GP）单克隆抗体（mab）均检测到抗原变异。一株分离株与抗-NC单克隆抗体P41呈阳性反应，该抗体迄今已知与极地狂犬病呈阳性反应。另一株分离株不与抗-NC单克隆抗体187.5反应；这是狂犬病病毒ERA/SAD株通常出现的反应。使用抗-GP单克隆抗体，可以根据分离株的来源地对其进行分组。来自高原州的分离株未被抗-GP单克隆抗体ERA 543和P44.7.2中和。所研究的分离株的糖蛋白抗原模式与尼日利亚用于犬免疫的低代鸡胚传代（LEP）Flury株疫苗病毒的模式不同。
