Botros B A, Salib A W, Mellick P W, Linn J M, Soliman A K, Scott R M
United States Naval Medical Research Unit No. 3, Cairo, Egypt.
J Med Virol. 1988 Feb;24(2):153-9. doi: 10.1002/jmv.1890240204.
Nineteen street rabies virus strains, isolated in Egypt from humans (two), dogs (nine), cats (two), farm animals (two), gerbils (three), and a jackal were antigenically analyzed. The Pasteur strain used for the preparation of human rabies vaccine, the Flury high and low egg passage stains (HEP, LEP) used for animal vaccines, and the challenge virus standard (CVS) strain were also assayed. All were examined by the indirect fluorescent antibody test, using a panel of 20 monoclonal antibodies against the nucleocapsid of rabies and rabies-related viruses. The rabies isolates demonstrated patterns of reactivity with the antinucleocapsid panel different from those of the Pasteur, HEP, and CVS strains. Representative human, dog, and rodent isolates were analyzed by neutralization tests in mice, with a second panel of 19 monoclonal antibodies against rabies and Mokola envelope glycoproteins. With this panel, the isolates demonstrated patterns of reactivity different from the vaccine strains. These data indicate antigenic variation between wild virus and vaccine strains.
对从埃及的人类（2例）、狗（9例）、猫（2例）、家畜（2例）、沙鼠（3例）以及一只胡狼身上分离出的19株街头狂犬病病毒毒株进行了抗原分析。还检测了用于制备人用狂犬病疫苗的巴斯德毒株、用于动物疫苗的弗吕里高代和低代鸡胚传代毒株（HEP、LEP）以及攻击病毒标准（CVS）毒株。使用一组针对狂犬病和狂犬病相关病毒核衣壳的20种单克隆抗体，通过间接荧光抗体试验对所有毒株进行检测。狂犬病分离株与抗核衣壳抗体组的反应模式不同于巴斯德、HEP和CVS毒株。通过在小鼠中进行中和试验，使用另一组针对狂犬病和莫科拉包膜糖蛋白的19种单克隆抗体，对具有代表性的人、狗和啮齿动物分离株进行分析。使用该抗体组时，分离株表现出与疫苗毒株不同的反应模式。这些数据表明野生病毒和疫苗毒株之间存在抗原变异。
