Department of Veterinary Medicine and Animal Productions, Naples University Federico II, Naples, Italy.
Biomed Res Int. 2013;2013:814635. doi: 10.1155/2013/814635. Epub 2013 Jun 5.
This report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. Approximately 80% were hemangiomas and angiosarcomas. Hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. Although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequent malignant vascular tumors. Hemangiopericytoma and tumors of muscle and soft connective tissue origin, alone and/or in association with tumor-like lesions, were less frequently seen. Furthermore, forty-five cases of intravascular papillary endothelial hyperplasia (IPEH), a lesion not previously reported in the urinary bladder of cattle, were also described. Bovine papillomavirus type-2 DNA was amplified in tumor samples. Forty vascular tumors were investigated by dual-labeling immunofluorescence, and, for the first time, a coexpression of E5 and platelet-derived growth factor β receptor (PDGF β R) was shown to occur. The results show that the BPV-2 E5 oncoprotein binds to the activated form of the PDGF β receptor thus playing an important role in mesenchymal as well as epithelial carcinogenesis of the urinary bladder. Furthermore, these findings demonstrate that BPV-2 infects both epithelial and mesenchymal cells.
本报告描述了在富含蕨类植物的土地上放牧的牛的 253 例膀胱间叶性肿瘤的组织病理学。其中约 80%为血管瘤和血管肉瘤。血管瘤（毛细血管型、海绵状和大血管型）是最常见的间叶性肿瘤，比血管肉瘤更为常见。尽管内皮细胞的形态可能有很大差异，但上皮样血管肉瘤，常含有多核细胞，是最常见的恶性血管肿瘤。血管外皮细胞瘤和源自肌肉和软结缔组织的肿瘤，单独或与肿瘤样病变一起，较少见。此外，还描述了 45 例以前在牛的膀胱中未报道过的血管内乳头状内皮增生（IPEH）病变。在肿瘤样本中扩增了牛乳头瘤病毒 2 型 DNA。通过双重标记免疫荧光法研究了 40 个血管肿瘤，首次显示 E5 和血小板衍生生长因子 β 受体（PDGF β R）的共表达。结果表明，BPV-2 E5 癌蛋白与 PDGF β 受体的激活形式结合，从而在膀胱的间叶性和上皮性癌发生中发挥重要作用。此外，这些发现表明 BPV-2 感染了上皮细胞和间叶细胞。
