State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
ACS Appl Mater Interfaces. 2013 Aug 28;5(16):8008-17. doi: 10.1021/am402089w. Epub 2013 Aug 1.
To promote and understand the biological responses of the implant via nanostructured surface design is essential for the development of bioactive bone implants. However, the control of the surface topography of the bioceramics in nanoscale is a big challenge because of their brittle property. Herein, the hydroxyapatite (HAp) bioceramics with distinct nanostructured topographies were fabricated via hydrothermal treatment using α-tricalcium phosphate ceramic as hard-template under different reaction conditions. HAp bioceramics with nanosheet, nanorod and micro-nanohybrid structured surface in macroscopical size were obtained by controlling the composition of the reaction media. Comparing with the traditional sample with flat and dense surface, the fabricated HAp bioceramics with hierarchical 3D micro-nanotextured surfaces possessed higher specific surface area, which selectively enhanced adsorption of specific proteins including Fn and Vn in plasma, and stimulated osteoblast adhesion, growth, and osoteogenic differentiation. In particular, the biomimetic features of the hierarchical micro-nanohybrid surface resulted in the best ability for simultaneous enhancement of protein adsorption, osteoblast proliferation, and differentiation. The results suggest that the hierarchical micro-nanohybrid topography might be one of the critical factors to be considered in the design of functional bone grafts.
为了促进和理解通过纳米结构表面设计的植入物的生物响应，对于生物活性骨植入物的发展是至关重要的。然而，由于生物陶瓷的脆性，控制其纳米级的表面形貌是一个巨大的挑战。在此，通过水热处理，使用α-磷酸三钙陶瓷作为硬模板，在不同的反应条件下，制备出具有明显纳米形貌的羟基磷灰石（HAp）生物陶瓷。通过控制反应介质的组成，可以获得具有纳米片、纳米棒和微纳混合结构表面的宏观尺寸的 HAp 生物陶瓷。与具有平坦致密表面的传统样品相比，具有分级 3D 微纳结构表面的 HAp 生物陶瓷具有更高的比表面积，其可以选择性地增强包括 Fn 和 Vn 在内的特定蛋白质在血浆中的吸附，并刺激成骨细胞的黏附、生长和成骨分化。特别是，分级微纳混合表面的仿生特征导致其具有同时增强蛋白质吸附、成骨细胞增殖和分化的最佳能力。研究结果表明，分级微纳混合形貌可能是设计功能性骨移植物时需要考虑的关键因素之一。
