Wang Yongmei, Wang Fang, Gong Min, Chen Lidan, Wang Yun, Xu Pu, Zeng Zhu, Hu Zuquan, Chen Jin
Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang 561113, China.
J Funct Biomater. 2025 May 8;16(5):165. doi: 10.3390/jfb16050165.
Constructing the surface structures of hydroxyapatite (HA) materials is a promising strategy for orchestrating the cell behaviors of bone marrow mesenchymal stem cells (BMSCs), beneficial for advancing BMSC-based tissue repair and regenerative therapies. The majority of previous strategies have focused on fabricating artificial micro-/nano-scale geometric topographies or patterns on HA surfaces. Yet, constructing surface crystal defects has received insufficient attention and application, despite their importance as highlighted by theoretical calculations. This is largely due to the instability of crystal defects, which tend to be eliminated during crystallization. Here, given the fact that stepped structures are rich in stable crystal defects along their edges and kinks, we crafted HA dishes featuring stepped surfaces and utilized them to establish cell culture models of BMSCs. The outcomes revealed that the stepped structures markedly altered the physicochemical properties of HA surfaces and affected the cytoskeleton structures, spreading area, cell morphology, and focal adhesions of BMSCs in the cell culture model, resulting in inhibited cell adhesion. Given that YAP is a key mechanical sensitive factor, and its nuclear translocation is closely tied to cytoskeletal reorganization, the nuclear translocation efficiency of YAP has been investigated. The results showed that a changed cell adhesion could affect the nuclear translocation efficiency of YAP, which would be an important reason for the change in proliferation and differentiation ability of BMSCs. This work not only enhances the understanding of the responses of BMSCs to HA surface structures but also facilitates the design and optimization of HA materials. Moreover, our manufacturing method is facile and efficient, positioning it to potentially integrate with other processing techniques for the more effective and precise regulation of BMSCs.
构建羟基磷灰石（HA）材料的表面结构是调控骨髓间充质干细胞（BMSC）细胞行为的一种有前景的策略，有利于推进基于BMSC的组织修复和再生治疗。以往的大多数策略都集中在在HA表面制造人工微/纳米级几何形貌或图案。然而，构建表面晶体缺陷尽管在理论计算中已被强调其重要性，但受到的关注和应用却不足。这主要是由于晶体缺陷的不稳定性，它们在结晶过程中往往会被消除。在此，鉴于阶梯结构沿其边缘和扭结处富含稳定的晶体缺陷，我们制作了具有阶梯表面的HA培养皿，并利用它们建立了BMSC的细胞培养模型。结果表明，阶梯结构显著改变了HA表面的物理化学性质，并影响了细胞培养模型中BMSC的细胞骨架结构、铺展面积、细胞形态和粘着斑，导致细胞粘附受到抑制。鉴于YAP是一个关键的机械敏感因子，其核转位与细胞骨架重组密切相关，因此研究了YAP的核转位效率。结果表明，细胞粘附的改变会影响YAP的核转位效率，这将是BMSC增殖和分化能力改变的一个重要原因。这项工作不仅增进了对BMSC对HA表面结构反应的理解，还促进了HA材料的设计和优化。此外，我们的制造方法简便高效，有可能与其他加工技术相结合，以更有效、精确地调控BMSC。
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