Arip Mohamad Nasir Mat, Heng Lee Yook, Ahmad Musa, Hasbullah Siti Aishah
Forest Products Division, Forest Research Institute Malaysia, Selangor DE 52109, Malaysia.
Faculty of Science and Technology/South East Asia Disaster Prevention Research Institute (SEADPRI), Universiti Kebangsaan Malaysia, Bangi, Selangor DE 43600, Malaysia.
Chem Cent J. 2013 Jul 16;7:122. doi: 10.1186/1752-153X-7-122. eCollection 2013.
A novel optical sensor for the rapid and direct determination of permethrin preservatives in treated wood was designed. The optical sensor was fabricated from the immobilisation of 2,6-dichloro-p-benzoquinone-4-chloroimide (Gibbs reagent) in nafion/sol-gel hybrid film and the mode of detection was based on absorption spectrophotometry. Physical entrapment was employed as a method of immobilisation.
The sensor gave a linear response range of permethrin between 2.56-383.00 μM with detection limit of 2.5 μM and demonstrated good repeatability with relative standard deviation (RSD) for 10 μM at 5.3%, 100 μM at 2.7%, and 200 μM at 1.8%. The response time of the sensor was 40 s with an optimum response at pH 11.
The sensor was useful for rapid screening of wood or treated wood products before detailed analysis using tedious procedure is performed. The validation study of the optical sensor against standard method HPLC successfully showed that the permethrin sensor tended to overestimate the permethrin concentration determined.
设计了一种用于快速直接测定经处理木材中氯菊酯防腐剂的新型光学传感器。该光学传感器是通过将2,6-二氯对苯醌-4-氯亚胺（吉布斯试剂）固定在全氟磺酸/溶胶-凝胶混合膜中制成的，检测模式基于吸收分光光度法。采用物理包埋作为固定化方法。
该传感器对氯菊酯的线性响应范围为2.56 - 383.00 μM，检测限为2.5 μM，并且在10 μM时相对标准偏差（RSD）为5.3%，100 μM时为2.7%，200 μM时为1.8%，显示出良好的重复性。该传感器的响应时间为40秒，在pH 11时响应最佳。
在使用繁琐程序进行详细分析之前，该传感器可用于快速筛选木材或经处理的木制品。光学传感器相对于标准方法高效液相色谱法（HPLC）的验证研究成功表明，氯菊酯传感器往往会高估所测定的氯菊酯浓度。
