Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Beijing 102206, China.
Food Chem. 2013 Dec 1;141(3):1645-50. doi: 10.1016/j.foodchem.2013.04.113. Epub 2013 May 9.
Soybean protein hydrolysates (SPHs) can bind calcium in order to form soluble peptide-calcium complexes. However, amino acid composition and structural characteristics of the calcium chelating SPHs are still unclear. This study separated SPHs with calcium and iron immobilized metal affinity chromatography (IMAC), and examined the effects of SPHs with different amino acid composition on calcium binding capacity. Three fractions (FFe-1, FFe-2 and FFe-3) isolated with IMAC-Fe(3+) were shown possessing increased Glu, Gln, Lys and Pro content from FFe-1 to FFe-3, and improved amount of bound calcium. Furthermore, the fractions adsorbed on IMAC-Ca(2+) (Fe(3+)) were separated and identified with reverse-phase (RP)-HPLC and MALDI-TOF MS/MS. The results showed that the sequence of peptides from FCa-2 and FFe-3 fractions was DEGEQPRPFPFP.
大豆蛋白水解物 (SPHs) 可以与钙结合形成可溶性肽-钙复合物。然而，与钙结合的 SPHs 的氨基酸组成和结构特征仍不清楚。本研究采用钙和铁固定化金属亲和层析（IMAC）分离 SPHs，并研究了不同氨基酸组成的 SPHs 对钙结合能力的影响。IMAC-Fe(3+) 分离得到的三个组分（FFe-1、FFe-2 和 FFe-3）的 Glu、Gln、Lys 和 Pro 含量逐渐增加，结合钙的量也随之增加。此外，用反相（RP）-HPLC 和 MALDI-TOF MS/MS 对吸附在 IMAC-Ca(2+)（Fe(3+)）上的组分进行了分离和鉴定。结果表明，FCa-2 和 FFe-3 组分的肽序列为 DEGEQPRPFPFP。
