鸡豆和兵豆益生元提取物及鸭蛋蛋白粉经羊膜内给药对钙状态和肠道功能的影响。
Intra-Amniotic Administration (Gallus gallus) of Cicer arietinum and Lens culinaris Prebiotics Extracts and Duck Egg White Peptides Affects Calcium Status and Intestinal Functionality.
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Department of Food Science and Technology, Cornell University, Ithaca, NY 14850, USA.
出版信息
Nutrients. 2017 Jul 21;9(7):785. doi: 10.3390/nu9070785.
Calcium (Ca) is one of the most abundant inorganic elements in the human body and has many important physiological roles. Prebiotics and bioactive peptides are two important substances used to promote calcium uptake. However, the difference in mechanisms of the calcium uptake from these two supplements is not clear. By using the model and the intra-amniotic administration procedure, the aim of this study was to investigate whether Ca status, intestinal functionality, and health-promoting bacterial populations were affected by prebiotics extracted from chickpea and lentil, and duck egg white peptides (DPs). Eleven groups (non-injected; 18 MΩ H₂O; 4 mmol/L CaCl₂; 50 mg/mL chickpea + 4 mmol/L CaCl₂; 50 mg/mL lentil + 4 mmol/L CaCl₂; 40 mg/mL DPs + 4 mmol/L CaCl₂; 5 mg/mL Val-Ser-Glu-Glu (VSEE) + 4 mmol/L CaCl₂; 50 mg/mL chickpea; 50 mg/mL lentil; 40 mg/mL DPs; 5 mg/mL VSEE) were utilized. Upon hatch, blood, cecum, small intestine, liver and bone were collected for assessment of serum bone alkaline phosphate level (BALP), the relative abundance of intestinal microflora, expression of Ca-related genes, brush border membrane (BBM) functional genes, and liver and bone mineral levels, respectively. The BALP level increased in the presence of lentil, DPs and VSEE ( < 0.05). The relative abundance of probiotics increased significantly ( < 0.05) by VSEE + Ca and chickpea. The expression of CalbindinD9k (Ca transporter) increased ( < 0.05) in Ca, chickpea + Ca and lentil + Ca groups. In addition, the brush border membrane functionality genes expressions increased ( < 0.05) by the chickpea or lentil extracts. Prebiotics and DPs beneficially affected the intestinal microflora and duodenal villus surface area. This research expands the understanding of the prebiotics' properties of chickpea and lentil extracts, and peptides' effects on calcium metabolism and gut health.
钙（Ca）是人体中含量最丰富的无机元素之一，具有许多重要的生理作用。益生元和生物活性肽是两种促进钙吸收的重要物质。然而，这两种补充剂的钙吸收机制不同。本研究采用模型和羊膜内给药程序，旨在研究鹰嘴豆和扁豆提取物以及鸭蛋清肽（DPs）是否会影响钙状态、肠道功能和促进健康的细菌种群。共设置 11 个组（未注射；18 MΩ H₂O；4 mmol/L CaCl₂；50 mg/mL 鹰嘴豆+4 mmol/L CaCl₂；50 mg/mL 扁豆+4 mmol/L CaCl₂；40 mg/mL DPs+4 mmol/L CaCl₂；5 mg/mL Val-Ser-Glu-Glu（VSEE）+4 mmol/L CaCl₂；50 mg/mL 鹰嘴豆；50 mg/mL 扁豆；40 mg/mL DPs；5 mg/mL VSEE）。出壳后，采集血液、盲肠、小肠、肝脏和骨骼，用于评估血清骨碱性磷酸酶水平（BALP）、肠道微生物群落的相对丰度、钙相关基因的表达、刷状缘膜（BBM）功能基因以及肝脏和骨骼矿物质水平。存在扁豆、DPs 和 VSEE 时，BALP 水平升高（<0.05）。VSEE+Ca 和鹰嘴豆使益生菌的相对丰度显著增加（<0.05）。CalbindinD9k（钙转运蛋白）的表达在 Ca、鹰嘴豆+Ca 和扁豆+Ca 组中增加（<0.05）。此外，刷状缘膜功能基因的表达通过鹰嘴豆或扁豆提取物增加（<0.05）。益生元和 DPs 有益地影响了肠道微生物群和十二指肠绒毛表面积。这项研究扩展了对鹰嘴豆和扁豆提取物的益生元特性以及肽对钙代谢和肠道健康的影响的理解。
Zotero 插件