College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Food Chem. 2013 Dec 1;141(3):3085-92. doi: 10.1016/j.foodchem.2013.05.145. Epub 2013 Jun 10.
A study of an enzymatic method for the production of galactosylglycerol is described. The effects of enzyme sources, enzyme amount, reaction temperature, reaction time, substrate ratio of glycerol to galactose, buffer content and pH on galactosylglycerol yield (mg/ml) were investigated. Under the optimum reaction conditions of β-galactosidases from Kluyveromyces lactis 240 U/ml, temperature 40 °C, time 24 h, buffer amount 60% (percent volume of buffer to that of substrates, pH 6.5), and the substrate molar ratio of 10 (glycerol16 mmol:galactose 1.6 mmol), the yield of galactosylglycerol was up to 116.47 mg/ml (galactose conversion 55.88%). The product was purified by activated charcoal and Sephadex G-15 column chromatography, up to 96%. The purified galactosylglycerol was fully characterised by MS and NMR, and identified as a mixture of (2R)- and (2S)- 3-O-β-D-galactopyranosyl-glycerol.
本文研究了一种酶法生产半乳糖基甘油的方法。考察了酶源、酶用量、反应温度、反应时间、甘油与半乳糖的底物比、缓冲液含量和 pH 值对半乳糖基甘油产率（mg/ml）的影响。在最适反应条件下，即乳糖酶 Kluyveromyces lactis 240 U/ml、温度 40°C、时间 24 h、缓冲液用量 60%（缓冲液体积与底物体积之比，pH6.5）、底物摩尔比 10（甘油 16mmol：半乳糖 1.6mmol），半乳糖基甘油的产率最高可达 116.47mg/ml（半乳糖转化率 55.88%）。产物通过活性炭和 Sephadex G-15 柱层析进行纯化，纯度可达 96%。通过 MS 和 NMR 对纯化的半乳糖基甘油进行了充分表征，鉴定为（2R）-和（2S）-3-O-β-D-半乳糖吡喃糖苷甘油的混合物。
