Martinez-Garcia Marta, van der Maarel Marc J E C
Aquatic Biotechnology and Bioproduct Engineering, Engineering and Technology Institute Groningen (ENTEG), University of Groningen, Groningen, The Netherlands.
AMB Express. 2016 Dec;6(1):71. doi: 10.1186/s13568-016-0244-6. Epub 2016 Sep 13.
Floridoside is a compatible solute synthesized by red algae that has attracted considerable attention due to its promising antifouling and therapeutic properties. However, research on industrial applications of floridoside is hampered by limited compound availability and the development of a production process yielding high amounts of this glycoside has not been explored yet. In the present work, floridoside accumulation by the red microalgae Galdieria sulphuraria under different conditions was investigated in order to optimize the production of this glycoside in this microalgae. G. sulphuraria shows consider advantages over other red algae as potential industrial producer of floridoside due to its unicellular nature, its ability to grow heterotrophically in complete darkness and its acidophilic lifestyle. The main compatible solute accumulated by G. sulphuraria under salt stress was purified, identified as floridoside by (1)H-NMR and used as standard for quantification. Our results showed that applying the osmotic stress after the cells had grown first in medium with no salt resulted in higher floridoside yields compared to those obtained in cells growing under osmotic stress from the beginning. Among several parameters tested, the use of glycerol as carbon source for cell growth showed the most significant impact on floridoside accumulation, which reached a maximum of 56.8 mg/g dry biomass.
弗罗里多苷是一种由红藻合成的相容性溶质，因其具有潜在的防污和治疗特性而备受关注。然而，弗罗里多苷的工业应用研究受到化合物可得性有限的阻碍，并且尚未探索出一种能大量生产这种糖苷的生产工艺。在本研究中，研究了红色微藻嗜硫热栖菌在不同条件下弗罗里多苷的积累情况，以优化该微藻中这种糖苷的生产。嗜硫热栖菌作为弗罗里多苷潜在的工业生产者，相较于其他红藻具有显著优势，因为它具有单细胞性质、能够在完全黑暗中异养生长以及嗜酸的生活方式。嗜硫热栖菌在盐胁迫下积累的主要相容性溶质经纯化后，通过¹H-NMR鉴定为弗罗里多苷，并用作定量标准。我们的结果表明，与从一开始就在渗透胁迫下生长的细胞相比，先在无盐培养基中生长的细胞在施加渗透胁迫后能获得更高的弗罗里多苷产量。在测试的几个参数中，使用甘油作为细胞生长的碳源对弗罗里多苷的积累影响最为显著，其产量最高可达56.8毫克/克干生物量。
