Department of Orthopaedic Surgery, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Japan, 980-8574.
Muscle Nerve. 2014 May;49(5):700-8. doi: 10.1002/mus.23950. Epub 2014 Feb 27.
The active form of vitamin D (1,25-dihydroxy-vitamin D3) is known to increase fast-type myosin heavy chain expression in differentiated myogenic cell lines. The mechanisms for this effect are not fully understood. The aim of this study was to determine the role of signals transmitted through the vitamin D receptor (VDR) during differentiation of myoblasts.
Electroporation was used to introduce VDR siRNA molecules into C2C12 and G8 murine myoblast cell lines. Gene and protein expression profiles of VDR-gene silenced cells were analyzed in vitro.
Suppressing VDR expression by RNA interference resulted in inhibition of myogenic differentiation of C2C12 and G8 cell lines at both mRNA and protein levels.
Our results suggest that myoblasts require signals transmitted through VDR for differentiation into myocytes and emphasize the importance of VDR expression in skeletal muscles for maintaining muscle volume in the elderly.
活性维生素 D（1,25-二羟维生素 D3）已知可增加分化肌细胞系中的快速型肌球蛋白重链表达。其作用机制尚未完全阐明。本研究旨在确定维生素 D 受体（VDR）在成肌细胞分化过程中传递的信号的作用。
电穿孔用于将 VDR siRNA 分子引入 C2C12 和 G8 鼠成肌细胞系。在体外分析 VDR 基因沉默细胞的基因和蛋白质表达谱。
通过 RNA 干扰抑制 VDR 表达导致 C2C12 和 G8 细胞系在 mRNA 和蛋白质水平上的成肌分化受到抑制。
我们的结果表明，成肌细胞需要通过 VDR 传递的信号才能分化为肌细胞，并强调 VDR 在骨骼肌中的表达对于维持老年人的肌肉量的重要性。
