Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, USA.
Poult Sci. 2013 Aug;92(8):2201-11. doi: 10.3382/ps.2012-03004.
Numerous vaccination strategies have been evaluated to develop effective vaccines against Campylobacter jejuni colonization in poultry but with limited success. The following experiments were conducted to investigate the effect of biodegradable and biocompatible poly (lactide-co-glycolide) nanoparticle (NP) encapsulated outer membrane proteins (OMP) of C. jejuni. Chickens were vaccinated with different routes [subcutaneous (s/c) or oral] and doses (25, 125, or 250 µg) of candidate nanoparticle vaccine with appropriate control groups. Serum and cloacal fecal samples were taken at regular intervals of time, and the birds were euthanized 7 d postchallenge with C. jejuni. The results were interpreted based on anti-OMP immunoglobulin response in chicken and intestinal colonization of C. jejuni. The C. jejuni colonization in cecal and cloacal contents at 7 d postchallenge was below the detection limit in the s/c vaccinated groups, but the other groups demonstrated varying degrees of colonization. The serum IgA was higher in the group vaccinated s/c with OMP only compared with the rest of the groups. The serum- and fecal-IgY titers were consistently higher in the s/c vaccinated groups (with or without NP) than the rest of the groups. Elevated levels of OMP specific serum antibodies correlated with below the limit of detection levels of Campylobacter colonization in broiler chickens receiving 125 μg of OMP alone and the OMP+NP vaccine s/c. In conclusion, the s/c route of vaccination with or without NP encapsulated OMP of C. jejuni may serve as a candidate vaccine for control of C. jejuni colonization in chickens.
已经评估了许多疫苗接种策略，以开发针对家禽中空肠弯曲菌定植的有效疫苗，但效果有限。进行了以下实验来研究可生物降解和生物相容的聚（乳酸-共-乙醇酸）纳米颗粒（NP）包封的空肠弯曲菌外膜蛋白（OMP）的效果。鸡通过不同途径[皮下（s/c）或口服]和候选纳米颗粒疫苗的剂量（25、125 或 250 µg）进行接种，并设立适当的对照组。定期采集血清和泄殖腔粪便样本，在接种空肠弯曲菌后 7 天对鸡进行安乐死。结果根据鸡的抗 OMP 免疫球蛋白反应和空肠弯曲菌的肠道定植进行解释。在接种后 7 天，s/c 接种组的盲肠和泄殖腔内容物中的空肠弯曲菌定植低于检测限，但其他组则表现出不同程度的定植。与其他组相比，仅 OMP 皮下接种组的血清 IgA 更高。s/c 接种组（有 NP 或无 NP）的血清和粪便 IgY 滴度均高于其他组。特异性 OMP 血清抗体水平升高与接受 125 µg 单独 OMP 和 OMP+NP 疫苗 s/c 的肉鸡中弯曲菌定植低于检测限水平相关。总之，s/c 途径接种 OMP 或 OMP 包封的 NP 可能成为控制鸡空肠弯曲菌定植的候选疫苗。
