Naguib Mostafa, Sharma Shreeya, Schneider Abigail, Wehmueller Sarah, Abdelaziz Khaled
Department of Animal and Veterinary Science, Clemson University, Clemson, SC 29634, USA.
Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Cairo 12211, Egypt.
Vaccines (Basel). 2024 Aug 10;12(8):908. doi: 10.3390/vaccines12080908.
This study was undertaken to evaluate and compare the efficacy of different multi-antigen vaccines, including heat-inactivated, whole lysate, and subunit (outer membrane proteins [OMPs]) vaccines along with the immunostimulant CpG ODN in controlling colonization in chickens. In the first trial, 125 μg of OMPs and 50 μg of CpG ODN were administered individually or in combination, either in ovo to chick embryos or subcutaneously (SC) to one-day-old chicks. In the second trial, different concentrations of antigens (heat-killed, whole lysate, and OMPs) were administered SC to one-day-old chicks. The results of the first trial revealed that SC immunization with the combination of CpG ODN and OMPs elevated interferon (IFN)-γ, interleukin (IL)-1β, and IL-13 gene expression in the spleen, significantly increased serum IgM and IgY antibody levels, and reduced cecal counts by approximately 1.2 log. In contrast, in ovo immunization did not elicit immune responses or confer protection against . The results of the second trial showed that SC immunization with whole lysate or 200 μg OMPs reduced counts by approximately 1.4 and 1.1 log, respectively. In conclusion, lysate and OMPs are promising vaccine antigens for reducing colonization in chickens.
本研究旨在评估和比较不同多抗原疫苗的效力，包括热灭活疫苗、全菌裂解物疫苗和亚单位（外膜蛋白[OMPs]）疫苗，以及免疫刺激剂CpG ODN在控制鸡群定植方面的效果。在第一次试验中，将125μg OMPs和50μg CpG ODN单独或联合给药，通过卵内注射给鸡胚，或皮下（SC）注射给1日龄雏鸡。在第二次试验中，将不同浓度的抗原（热灭活抗原、全菌裂解物和OMPs）皮下注射给1日龄雏鸡。第一次试验结果显示，用CpG ODN和OMPs联合进行皮下免疫可提高脾脏中干扰素（IFN）-γ、白细胞介素（IL）-1β和IL-13的基因表达，显著提高血清IgM和IgY抗体水平，并使盲肠菌数减少约1.2个对数。相比之下，卵内免疫未引发免疫反应或提供针对……的保护。第二次试验结果表明，用全菌裂解物或200μg OMPs进行皮下免疫分别使菌数减少约1.4和1.1个对数。总之，全菌裂解物和OMPs是减少鸡群定植的有前景的疫苗抗原。
