School of Biological Science, Flinders University, Adelaide, South Australia, Australia.
PLoS One. 2013 Jul 10;8(7):e67975. doi: 10.1371/journal.pone.0067975. Print 2013.
The coral pathogen, Vibrio coralliilyticus shows specific chemotactic search pattern preference for oxic and anoxic conditions, with the newly identified 3-step flick search pattern dominating the patterns used in oxic conditions. We analyzed motile V. coralliilyticus cells for behavioral changes with varying oxygen concentrations to mimic the natural coral environment exhibited during light and dark conditions. Results showed that 3-step flicks were 1.4× (P = 0.006) more likely to occur in oxic conditions than anoxic conditions with mean values of 18 flicks (95% CI = 0.4, n = 53) identified in oxic regions compared to 13 (95% CI = 0.5, n = 38) at anoxic areas. In contrast, run and reverse search patterns were more frequent in anoxic regions with a mean value of 15 (95% CI = 0.7, n = 46), compared to a mean value of 10 (95% CI = 0.8, n = 29) at oxic regions. Straight swimming search patterns remained similar across oxic and anoxic regions with a mean value of 13 (95% CI = 0.7, n = oxic: 13, anoxic: 14). V. coralliilyticus remained motile in oxic and anoxic conditions, however, the 3-step flick search pattern occurred in oxic conditions. This result provides an approach to further investigate the 3-step flick.
珊瑚病原体 Vibrio coralliilyticus 对好氧和缺氧条件表现出特定的趋化搜索模式偏好，新确定的 3 步弹击搜索模式主导好氧条件下使用的模式。我们分析了运动的 Vibrio coralliilyticus 细胞在不同氧浓度下的行为变化，以模拟自然珊瑚环境在光照和黑暗条件下表现出的变化。结果表明，3 步弹击在好氧条件下发生的可能性是缺氧条件下的 1.4 倍（P=0.006），在好氧区域识别到的平均 18 次弹击（95%置信区间 0.4，n=53）高于缺氧区域的 13 次（95%置信区间 0.5，n=38）。相比之下，在缺氧区域，跑和反向搜索模式更为频繁，平均 15 次（95%置信区间 0.7，n=46），而在好氧区域的平均值为 10 次（95%置信区间 0.8，n=29）。在好氧和缺氧区域，直线游泳搜索模式保持相似，平均值为 13 次（95%置信区间 0.7，n=oxic: 13，anoxic: 14）。Vibrio coralliilyticus 在好氧和缺氧条件下仍保持运动，但 3 步弹击搜索模式仅发生在好氧条件下。该结果提供了一种方法来进一步研究 3 步弹击。
