Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota, School of Medicine and Health Sciences, Grand Forks, ND 58202-9037, United States.
Neurosci Lett. 2013 Sep 13;551:23-7. doi: 10.1016/j.neulet.2013.06.058. Epub 2013 Jul 19.
Because α-synuclein (Snca) is involved in neuroinflammatory response, we determined if its expression altered blood-brain barrier (BBB) permeability. To induce increased BBB permeability, Snca gene-ablated (KO) and wild-type (WT) mice were injected (i.p.) with lipopolysaccharide (LPS). To assess changes in BBB permeability, Evans blue was injected (i.p.) and extravasation into the brain assessed using fluorescence spectroscopy. WT mice had a significant increase in BBB permeability at 1, 3, and 6h post-injection of LPS relative to untreated mice. Contrary to WT mice, LPS did not induce a time-dependent change in BBB permeability in KO mice. Although brain edema is associated with increased BBB permeability, no significant difference in edema was found between groups. These results show that Snca expression is associated with increased reactive opening of the BBB in response to LPS.
由于α-突触核蛋白（Snca）参与神经炎症反应，我们确定其表达是否改变了血脑屏障（BBB）的通透性。为了诱导 BBB 通透性增加，基因敲除（KO）和野生型（WT）小鼠被注射（腹腔内）脂多糖（LPS）。为了评估 BBB 通透性的变化，用 Evans 蓝（腹腔内）注射，并使用荧光光谱法评估脑内的漏出。与未处理的小鼠相比，WT 小鼠在 LPS 注射后 1、3 和 6 小时 BBB 通透性有显著增加。与 WT 小鼠相反，LPS 没有在 KO 小鼠中诱导 BBB 通透性的时间依赖性变化。尽管脑水肿与 BBB 通透性增加有关，但各组之间没有发现水肿有显著差异。这些结果表明，Snca 的表达与 LPS 反应中 BBB 的反应性开放增加有关。
