School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.
School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.
Colloids Surf B Biointerfaces. 2013 Nov 1;111:469-78. doi: 10.1016/j.colsurfb.2013.06.020. Epub 2013 Jun 28.
This work investigates the effect of mammalian cell culture conditions on 3D printed calcium phosphate scaffolds. The purpose of the studies presented was to characterise the changes in scaffold properties in physiologically relevant conditions. Differences in crystal morphologies were observed between foetal bovine serum-supplemented media and their unsupplemented analogues, but not for supplemented media containing tenocytes. Scaffold porosity was found to increase for all conditions studied, except for tenocyte-seeded scaffolds. The presence of tenocytes on the scaffold surface inhibited any increase in scaffold porosity in the presence of extracellular matrix secreted by the tenocytes. For acellular conditions the presence or absence of sera proteins strongly affected the rate of dissolution and the distribution of pore diameters within the scaffold. Exposure to high sera protein concentrations led to the development of significant numbers of sub-micron pores, which was otherwise not observed. The implication of these results for cell culture research employing calcium phosphate scaffolds is discussed.
这项工作研究了哺乳动物细胞培养条件对 3D 打印磷酸钙支架的影响。本研究的目的是在生理相关条件下表征支架性能的变化。在胎牛血清补充培养基和未补充的类似物之间观察到晶体形态的差异，但在含有肌腱细胞的补充培养基中则没有观察到。发现所有研究条件下的支架孔隙率都增加了，除了接种肌腱细胞的支架。在肌腱细胞存在于支架表面的情况下，即使存在由肌腱细胞分泌的细胞外基质，也会抑制支架孔隙率的增加。对于无细胞条件，血清蛋白的存在与否强烈影响支架内孔径的溶解速度和分布。暴露于高浓度血清蛋白会导致大量亚微米孔的形成，否则不会观察到这种情况。讨论了这些结果对使用磷酸钙支架进行细胞培养研究的影响。
