Pramanik Sumit, Ataollahi Forough, Pingguan-Murphy Belinda, Oshkour Azim Ataollahi, Osman Noor Azuan Abu
Centre for Applied Biomechanics, Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
Department of Mechanical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
Sci Rep. 2015 May 7;5:9806. doi: 10.1038/srep09806.
Scaffold design from xenogeneic bone has the potential for tissue engineering (TE). However, major difficulties impede this potential, such as the wide range of properties in natural bone. In this study, sintered cortical bones from different parts of a bovine-femur impregnated with biodegradable poly(ethylene glycol) (PEG) binder by liquid phase adsorption were investigated. Flexural mechanical properties of the PEG-treated scaffolds showed that the scaffold is stiffer and stronger at a sintering condition of 1000°C compared with 900°C. In vitro cytotoxicity of the scaffolds evaluated by Alamar Blue assay and microscopic tests on human fibroblast cells is better at 1000°C compared with that at 900°C. Furthermore, in vitro biocompatibility and flexural property of scaffolds derived from different parts of a femur depend on morphology and heat-treatment condition. Therefore, the fabricated scaffolds from the distal and proximal parts at 1000°C are potential candidates for hard and soft TE applications, respectively.
来自异种骨的支架设计具有组织工程（TE）的潜力。然而，一些主要困难阻碍了这种潜力的发挥，比如天然骨的性质范围广泛。在本研究中，对通过液相吸附法用可生物降解的聚乙二醇（PEG）粘合剂浸渍的牛股骨不同部位的烧结皮质骨进行了研究。经PEG处理的支架的弯曲力学性能表明，与900°C的烧结条件相比，在1000°C的烧结条件下支架更硬且更强。通过阿拉玛蓝测定法评估的支架体外细胞毒性以及对人成纤维细胞的显微镜测试表明，1000°C时的结果优于900°C时。此外，源自股骨不同部位的支架的体外生物相容性和弯曲性能取决于形态和热处理条件。因此，在1000°C下由远端和近端部位制成的支架分别是硬组织工程和软组织工程应用的潜在候选材料。
