Sarowska Jolanta, Olszak Tomasz, Jama-Kmiecik Agnieszka, Frej-Madrzak Magdalena, Futoma-Koloch Bozena, Gawel Andrzej, Drulis-Kawa Zuzanna, Choroszy-Krol Irena
Department of Basic Sciences, Faculty of Health Sciences, Wroclaw Medical University, Chalubinskiego 4, 50-368 Wroclaw, Poland.
Department of Pathogen Biology and Immunology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
Life (Basel). 2022 Jun 6;12(6):845. doi: 10.3390/life12060845.
The pathogenicity of many bacterial strains is determined by the acquisition of virulence genes and depends on many factors. The aim of this study was to analyse the phylogenetic background, virulence patterns, and drug susceptibility of 132 isolates tested in the context of the ExPEC (Extraintestinal Pathogenic ) pathotype and the correlation of these features with bacterial isolation source: food (retail meat), poultry farms (AFEC-Avian Faecal ), and patients with UTI (urinary tract infection) symptoms. The drug-susceptibility results of tested isolates obtained indicate that the resistance profile-ampicillin/tetracycline/trimethoprim+sulfamethoxazole/ciprofloxacin (AMP/TE/SXT/CIP)-was most frequently observed. The multidrug resistance (MDR) phenotype was found in 31.8% of isolates from poultry farms, 36.8% of strains isolated from food, and 20% of clinical samples. The greatest similarity of virulence profiles applied to isolates derived from poultry farms and food. Most of the AFEC from poultry farms and food-derived isolates belonged to commensals from phylogroups A and B1, while among the isolates from patients with UTI symptoms, the most common was the B2 phylogroup. The collective analysis showed similarity of the three studied groups of isolates in terms of the presented patterns of antimicrobial resistance, while the virulence profiles of the isolates studied showed great diversity. The phylogroup analysis showed no similarity between the poultry/food isolates and the UTI isolates, which had significant pathogenic potential.
许多细菌菌株的致病性由毒力基因的获得决定，并取决于多种因素。本研究的目的是分析在肠外致病性大肠杆菌（ExPEC）致病型背景下测试的132株分离株的系统发育背景、毒力模式和药敏性，以及这些特征与细菌分离来源（食品（零售肉类）、家禽养殖场（AFEC - 禽粪便）和有尿路感染（UTI）症状的患者）之间的相关性。所获得的受试分离株的药敏结果表明，最常观察到的耐药谱为氨苄西林/四环素/甲氧苄啶 + 磺胺甲恶唑/环丙沙星（AMP/TE/SXT/CIP）。在家禽养殖场分离株中，31.8%发现有多药耐药（MDR）表型，食品分离株中为36.8%，临床样本中为20%。应用于家禽养殖场和食品来源分离株的毒力谱最为相似。家禽养殖场的大多数AFEC和食品来源的分离株属于A和B1系统发育群的共生菌，而在有UTI症状患者的分离株中，最常见的是B2系统发育群。综合分析表明，在所呈现的抗菌药物耐药模式方面，三组受试分离株具有相似性，而受试分离株的毒力谱显示出很大的多样性。系统发育群分析表明，家禽/食品分离株与具有显著致病潜力的UTI分离株之间没有相似性。
