Exp Dermatol. 2013 Aug;22(8):558-60. doi: 10.1111/exd.12193.
Blue light is a UV-free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand- and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells (DC) are directly affected by blue light irradiation in vitro. Here, we report that irradiation neither induced apoptosis nor maturation of monocyte-derived and myeloid DC. However, subsequent DC maturation upon LPS/IFNγ stimulation was impaired in a dose-dependent manner as assessed by maturation markers and cytokine release. Moreover, the potential of this DC to induce cytokine secretion from allogeneic CD4 T cells was reduced. In conclusion, unlike UV irradiation, blue light irradiation at high and low doses only resulted in impaired DC maturation upon activation and a reduced subsequent stimulatory capacity in allogeneic MLRs with strongest effects at higher doses.
蓝光是一种无 UV 辐射的光，适用于治疗慢性皮肤炎症，如特应性皮炎、银屑病和手足湿疹。然而，其作用模式仍缺乏更好的理解。出于这个原因，我们研究了在体外情况下，树突状细胞（DC）是否会直接受到蓝光照射的影响。在这里，我们报告称，蓝光照射既不会诱导单核细胞来源的和髓样 DC 凋亡，也不会诱导其成熟。然而，随后用 LPS/IFNγ刺激时，DC 的成熟会被剂量依赖性地抑制，这可以通过成熟标志物和细胞因子释放来评估。此外，这种 DC 诱导同种异体 CD4 T 细胞分泌细胞因子的能力也降低了。总之，与 UV 照射不同，高剂量和低剂量的蓝光照射仅在激活后导致 DC 成熟受损，并且在同种异体 MLR 中的后续刺激能力降低，高剂量时的作用最强。
