从英国常春藤（Hedera helix L.）中分离和化学分析纳米颗粒。
Isolation and chemical analysis of nanoparticles from English ivy (Hedera helix L.).
机构信息
Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996, USA.
出版信息
J R Soc Interface. 2013 Jul 24;10(87):20130392. doi: 10.1098/rsif.2013.0392. Print 2013 Oct 6.
Bio-inspiration for novel adhesive development has drawn increasing interest in recent years with the discovery of the nanoscale morphology of the gecko footpad and mussel adhesive proteins. Similar to these animal systems, it was discovered that English ivy (Hedera helix L.) secretes a high strength adhesive containing uniform nanoparticles. Recent studies have demonstrated that the ivy nanoparticles not only contribute to the high strength of this adhesive, but also have ultraviolet (UV) protective abilities, making them ideal for sunscreen and cosmetic fillers, and may be used as nanocarriers for drug delivery. To make these applications a reality, the chemical nature of the ivy nanoparticles must be elucidated. In the current work, a method was developed to harvest bulk ivy nanoparticles from an adventitious root culture system, and the chemical composition of the nanoparticles was analysed. UV/visible spectroscopy, inductively coupled plasma mass spectrometry, Fourier transform infrared spectroscopy and electrophoresis were used in this study to identify the chemical nature of the ivy nanoparticles. Based on this analysis, we conclude that the ivy nanoparticles are proteinaceous.
近年来，随着对壁虎足底和贻贝粘合蛋白的纳米级形态的发现，对新型粘合材料的生物启发式研究引起了越来越多的关注。与这些动物系统类似，人们发现常春藤（Hedera helix L.）分泌出一种含有均匀纳米颗粒的高强度粘合剂。最近的研究表明，常春藤纳米颗粒不仅有助于提高这种粘合剂的强度，而且具有紫外线（UV）保护能力，使其成为理想的防晒霜和化妆品填充物，并且可以用作药物递送的纳米载体。为了使这些应用成为现实，必须阐明常春藤纳米颗粒的化学性质。在目前的工作中，开发了一种从不定根培养系统中收获大量常春藤纳米颗粒的方法，并对纳米颗粒的化学成分进行了分析。本研究采用紫外/可见分光光度法、电感耦合等离子体质谱法、傅里叶变换红外光谱法和电泳法来鉴定常春藤纳米颗粒的化学性质。基于此分析，我们得出结论，常春藤纳米颗粒是蛋白质。
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