Arisawa Y, Kubota T, Suto A, Kodaira S, Ishibiki K, Abe O
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Jpn J Surg. 1990 Jul;20(4):487-90. doi: 10.1007/BF02470838.
The ability of the host-immune defense mechanism of nude mice and their immunocompetent littermates to prevent liver metastases from the murine colon carcinoma, colon-26, was assessed. Give thousand tumor cells suspended in 0.05 ml of Hank's balanced salt solution were inoculated into the spleens of BALB/c nu/+ and BALB/c nu/nu mice. On the 21st day after inoculation, all the mice were sacrificed, and the liver metastases counted and the livers weighed. All the BALB/c nu/+ mice were found to have developed hepatic metastases with a mean of 10 nodules, whereas no hepatic metastases were observed in any of the 10 BALB/c nude mice. On the other hand, 4 of 6 nude mice developed hepatic metastases after treatment with anti-asialo GM1 antibody. These results indicate that the BALB/c nude mouse has an excellent host-immune defense mechanism for preventing liver metastasis, with NK cells in the liver and/or blood circulation perhaps playing an important role.
评估了裸鼠及其具有免疫能力的同窝仔鼠的宿主免疫防御机制预防小鼠结肠癌（结肠 -26）肝转移的能力。将悬浮于0.05 ml Hank平衡盐溶液中的1000个肿瘤细胞接种到BALB/c nu/+和BALB/c nu/nu小鼠的脾脏中。接种后第21天，处死所有小鼠，计数肝转移灶并称重肝脏。发现所有BALB/c nu/+小鼠均发生了肝转移，平均有10个结节，而10只BALB/c裸鼠中未观察到任何肝转移。另一方面，6只裸鼠中有4只在用抗去唾液酸GM1抗体治疗后发生了肝转移。这些结果表明，BALB/c裸鼠具有出色的宿主免疫防御机制来预防肝转移，肝脏和/或血液循环中的自然杀伤细胞可能起重要作用。
