Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160, USA.
J Am Coll Nutr. 2013;32(3):187-93. doi: 10.1080/07315724.2013.791167.
A simple method of using fingerstick blood glucose (FSBG) monitors to estimate blood ascorbate values after high-dose intravenous (IV) ascorbate infusion is evaluated as a substitution for high-performance liquid chromatography (HPLC) measurement.
In 33 participants, readings from FSBG monitors were taken before and after IV ascorbate infusions at various time points, with the postinfusion FSBG readings subtracted from the baseline glucose readings. The results of the subtractions (AAFSBG) were correlated with ascorbate concentrations detected by HPLC (AAHPLC).
A linear regression was found between ascorbate concentrations detected by the fingerstick method (AAFSBG) and by HPLC (AAHPLC). The linear correlations were identical in healthy subjects, diabetic subjects, and cancer patients. Analysis of variance obtained an AAFSBG/AAHPLC ratio of 0.90, with a 90% confidence interval of (0.69, 1.20). The corrections of AAFSBG improved similarity to AAHPLC but did not significantly differ from the uncorrected values.
The FSBG method can be used as an approximate estimation of high blood ascorbate concentration after IV ascorbate (>50 mg/dL, or 2.8 mM) without correction. However, this measurement is not accurate in detecting lower or baseline blood ascorbate. It is also important to highlight that in regard to glucose monitoring, FSBG readings will be erroneously elevated following IV ascorbate use and insulin should not be administered to patients based on these readings.
评估一种使用指尖血糖（FSBG）监测仪估计高剂量静脉（IV）给予抗坏血酸后血液抗坏血酸值的简单方法，作为高效液相色谱（HPLC）测量的替代方法。
在 33 名参与者中，在 IV 抗坏血酸输注的各个时间点之前和之后，从 FSBG 监测仪读取读数，将输注后的 FSBG 读数从基线葡萄糖读数中减去。减法的结果（AAFSBG）与 HPLC 检测到的抗坏血酸浓度（AAHPLC）相关。
在指尖法（AAFSBG）和 HPLC （AAHPLC）检测到的抗坏血酸浓度之间发现了线性回归。线性相关性在健康受试者、糖尿病患者和癌症患者中是相同的。方差分析得出 AAFSBG/AAHPLC 比值为 0.90，90%置信区间为（0.69，1.20）。AAFSBG 的校正提高了与 AAHPLC 的相似性，但与未校正值没有显著差异。
在没有校正的情况下，FSBG 法可用于 IV 抗坏血酸（>50mg/dL，或 2.8mM）后血液中高抗坏血酸浓度的近似估计。然而，这种测量方法在检测较低或基线血液抗坏血酸时并不准确。还需要强调的是，在葡萄糖监测方面，IV 抗坏血酸使用后 FSBG 读数会错误升高，不应根据这些读数给患者注射胰岛素。
