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一种果蝇核糖体蛋白在哺乳动物细胞中发挥作用。

A Drosophila ribosomal protein functions in mammalian cells.

作者信息

Maki C G, Rhoads D D, Diaz J J, Roufa D J

机构信息

Division of Biology, Kansas State University, Manhattan 66506.

出版信息

Mol Cell Biol. 1990 Sep;10(9):4524-8. doi: 10.1128/mcb.10.9.4524-4528.1990.

DOI:10.1128/mcb.10.9.4524-4528.1990
PMID:2388616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361039/
Abstract

A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.

摘要

将编码果蝇核糖体蛋白S14的cDNA表达载体转染到携带隐性RPS14依米丁抗性突变的培养中国仓鼠卵巢(CHO)细胞中。转化体合成了昆虫mRNA和多肽,因此表现出依米丁敏感表型。这些观察结果表明,昆虫蛋白被准确表达并正确组装成功能性的哺乳动物40S核糖体亚基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c6e/361039/7cf3356470ed/molcellb00045-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c6e/361039/5d1aacb62292/molcellb00045-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c6e/361039/7cf3356470ed/molcellb00045-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c6e/361039/5d1aacb62292/molcellb00045-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c6e/361039/7cf3356470ed/molcellb00045-0099-a.jpg

相似文献

1
A Drosophila ribosomal protein functions in mammalian cells.一种果蝇核糖体蛋白在哺乳动物细胞中发挥作用。
Mol Cell Biol. 1990 Sep;10(9):4524-8. doi: 10.1128/mcb.10.9.4524-4528.1990.
2
Genetic analysis of a vital mammalian housekeeping locus using CHO cells that express a transfected mutant allele.利用表达转染突变等位基因的中国仓鼠卵巢细胞(CHO细胞)对一个重要的哺乳动物管家基因座进行遗传分析。
Somat Cell Mol Genet. 1990 Nov;16(6):517-28. doi: 10.1007/BF01233092.
3
Synthesis and incorporation of human ribosomal protein S14 into functional ribosomes in human-Chinese hamster cell hybrids containing human chromosome 5: human RPS14 gene is the structural gene for ribosomal protein S14.人核糖体蛋白S14在含人5号染色体的人-中国仓鼠细胞杂种中合成并整合到功能性核糖体中:人RPS14基因是核糖体蛋白S14的结构基因。
Somat Cell Mol Genet. 1985 Nov;11(6):625-31. doi: 10.1007/BF01534727.
4
Ribosomal protein S14 is altered by two-step emetine resistance mutations in Chinese hamster cells.核糖体蛋白S14在中国仓鼠细胞中因两步吐根碱抗性突变而发生改变。
Mol Cell Biol. 1983 Feb;3(2):190-7. doi: 10.1128/mcb.3.2.190-197.1983.
5
The Chinese hamster cell emetine resistance gene. Analysis of cDNA and genomic sequences encoding ribosomal protein S14.中国仓鼠细胞吐根碱抗性基因。编码核糖体蛋白S14的cDNA和基因组序列分析。
J Biol Chem. 1983 Nov 10;258(21):13236-42.
6
Emetine resistance of Chinese hamster cells: structures of wild-type and mutant ribosomal protein S14 mRNAs.中国仓鼠细胞的吐根碱抗性:野生型和突变型核糖体蛋白S14 mRNA的结构
Mol Cell Biol. 1985 Jul;5(7):1655-9. doi: 10.1128/mcb.5.7.1655-1659.1985.
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A cloned human ribosomal protein gene functions in rodent cells.一个克隆的人类核糖体蛋白基因在啮齿动物细胞中发挥作用。
Mol Cell Biol. 1987 Oct;7(10):3767-74. doi: 10.1128/mcb.7.10.3767-3774.1987.
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Emetine resistance in chinese hamster ovary cells is associated with an altered ribosomal protein S14 mRNA.中国仓鼠卵巢细胞中的吐根碱抗性与核糖体蛋白S14 mRNA的改变有关。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1003-7. doi: 10.1073/pnas.79.4.1003.
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Emetine resistance in Chinese hamster cells is linked genetically with an altered 40S ribosomal subunit protein, S20.中国仓鼠细胞中的吐根碱抗性与一种改变的40S核糖体亚基蛋白S20存在遗传联系。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):415-9. doi: 10.1073/pnas.76.1.415.
10
Molecular evolution of the mammalian ribosomal protein gene, RPS14.哺乳动物核糖体蛋白基因RPS14的分子进化
Mol Biol Evol. 1991 Jul;8(4):503-14. doi: 10.1093/oxfordjournals.molbev.a040665.

引用本文的文献

1
A mammalian origin of bidirectional DNA replication within the Chinese hamster RPS14 locus.中国仓鼠RPS14基因座内双向DNA复制的哺乳动物起源。
Mol Cell Biol. 1994 Sep;14(9):5628-35. doi: 10.1128/mcb.14.9.5628-5635.1994.
2
Fine-structure map of the human ribosomal protein gene RPS14.人类核糖体蛋白基因RPS14的精细结构图谱
Mol Cell Biol. 1992 Apr;12(4):1680-6. doi: 10.1128/mcb.12.4.1680-1686.1992.

本文引用的文献

1
Differences in electrophoretic behaviour of eight ribosomal proteins from rat and rabbit tissues and evidence for proteolytic action on liver proteins.大鼠和兔组织中八种核糖体蛋白的电泳行为差异以及肝脏蛋白受蛋白水解作用的证据。
Mol Gen Genet. 1980;179(1):89-101. doi: 10.1007/BF00268450.
2
Linkage of genetic markers emt and chr in Chinese hamster cells.中国仓鼠细胞中遗传标记emt和chr的连锁
Somatic Cell Genet. 1980 Mar;6(2):215-224. doi: 10.1007/BF01538797.
3
Biochemical evidence for multiple independent emetine resistance genes in Chinese hamster cells.
中国仓鼠细胞中多个独立的吐根碱抗性基因的生化证据。
Mol Cell Biol. 1983 Feb;3(2):198-202. doi: 10.1128/mcb.3.2.198-202.1983.
4
Ribosomal protein S14 is altered by two-step emetine resistance mutations in Chinese hamster cells.核糖体蛋白S14在中国仓鼠细胞中因两步吐根碱抗性突变而发生改变。
Mol Cell Biol. 1983 Feb;3(2):190-7. doi: 10.1128/mcb.3.2.190-197.1983.
5
The Chinese hamster cell emetine resistance gene. Analysis of cDNA and genomic sequences encoding ribosomal protein S14.中国仓鼠细胞吐根碱抗性基因。编码核糖体蛋白S14的cDNA和基因组序列分析。
J Biol Chem. 1983 Nov 10;258(21):13236-42.
6
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.一对用于选择双酶切限制片段任一条DNA链的新型M13载体。
Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
7
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.利用处于SV40早期区域启动子控制下的细菌基因将哺乳动物细胞转化为抗生素抗性细胞。
J Mol Appl Genet. 1982;1(4):327-41.
8
Emetine resistance in chinese hamster ovary cells is associated with an altered ribosomal protein S14 mRNA.中国仓鼠卵巢细胞中的吐根碱抗性与核糖体蛋白S14 mRNA的改变有关。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1003-7. doi: 10.1073/pnas.79.4.1003.
9
Synthesis and incorporation of human ribosomal protein S14 into functional ribosomes in human-Chinese hamster cell hybrids containing human chromosome 5: human RPS14 gene is the structural gene for ribosomal protein S14.人核糖体蛋白S14在含人5号染色体的人-中国仓鼠细胞杂种中合成并整合到功能性核糖体中:人RPS14基因是核糖体蛋白S14的结构基因。
Somat Cell Mol Genet. 1985 Nov;11(6):625-31. doi: 10.1007/BF01534727.
10
Emetine resistance of Chinese hamster cells: structures of wild-type and mutant ribosomal protein S14 mRNAs.中国仓鼠细胞的吐根碱抗性:野生型和突变型核糖体蛋白S14 mRNA的结构
Mol Cell Biol. 1985 Jul;5(7):1655-9. doi: 10.1128/mcb.5.7.1655-1659.1985.