Maki C G, Rhoads D D, Diaz J J, Roufa D J
Division of Biology, Kansas State University, Manhattan 66506.
Mol Cell Biol. 1990 Sep;10(9):4524-8. doi: 10.1128/mcb.10.9.4524-4528.1990.
A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.
将编码果蝇核糖体蛋白S14的cDNA表达载体转染到携带隐性RPS14依米丁抗性突变的培养中国仓鼠卵巢(CHO)细胞中。转化体合成了昆虫mRNA和多肽,因此表现出依米丁敏感表型。这些观察结果表明,昆虫蛋白被准确表达并正确组装成功能性的哺乳动物40S核糖体亚基。