Nielsen-Smith K, McGill S, Frahm M, Roufa D J
Mol Cell Biol. 1983 Feb;3(2):198-202. doi: 10.1128/mcb.3.2.198-202.1983.
Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the ribosomes of the cells directly. Although mutations in a third gene, emtC, affect the translation apparatus of Chinese hamster peritoneal cells in vitro (Wasmuth et al., Mol. Cell. Biol. 1:58-65, 1981), the molecular product of the emtC locus remains to be determined. To study the molecular basis for genetic complementation among emetine-resistant Chinese hamster cell mutants, we analyzed ribosomal proteins elaborated by complementing, emetine-sensitive hybrid clones (EmtB X EmtA and EmtB X EmtC) and by emetine-resistant clones that segregated from the hybrids. The electrophoretic forms of ribosomal protein S14 (the emtB gene product) elaborated by these clones indicated that the EmtA and EmtC phenotypes are independent of the emtB locus and that the emtA and emtC loci are not chromosomally linked to emtB.
杂交互补研究表明，多个基因发生突变可使中国仓鼠细胞对生物碱翻译抑制剂吐根碱产生抗性。分别在中国仓鼠肺细胞系和卵巢细胞系中鉴定出的两个基因，即emtA和emtB，已知会直接影响细胞的核糖体。虽然第三个基因emtC的突变在体外会影响中国仓鼠腹膜细胞的翻译装置（瓦斯穆特等人，《分子与细胞生物学》1:58 - 65，1981年），但emtC基因座的分子产物仍有待确定。为了研究吐根碱抗性中国仓鼠细胞突变体之间遗传互补的分子基础，我们分析了由互补的、对吐根碱敏感的杂交克隆（EmtB×EmtA和EmtB×EmtC）以及从杂交体中分离出的对吐根碱抗性的克隆所产生的核糖体蛋白。这些克隆所产生的核糖体蛋白S14（emtB基因产物）的电泳形式表明，EmtA和EmtC表型独立于emtB基因座，并且emtA和emtC基因座在染色体上不与emtB连锁。
