Madjar J J, Traut R R
Mol Gen Genet. 1980;179(1):89-101. doi: 10.1007/BF00268450.
Divergence exists between eight ribosomal proteins of rat and rabbit. This is the first time such a divergence has been precisely demonstrated among mammals. In addition, a proteolytic activity, giving the appearance of modified proteins, is observed in the liver of both species but not in rabbit reticulocytes. These results were made possible by a recently developed method. Ribosomal proteins were compared by two-dimensional polyacrylamide gel electrophoresis in four different but related systems. The precise position of each individual protein was established in each of the four systems. Certain ribosomal proteins from different tissues, seemingly identical in one system were found to be different in other systems. Significant differences occurred between proteins of each ribosomal subunit from the two different species. Variation between reticulocyte and liver ribosomal proteins of the rabbit were minor. Several liver proteins of both species change position or disappear and apparently new proteins appear, if appropriate steps are not taken to prevent proteolysis. Differences in behavior of three small subunit and five large subunit proteins are attributable to an evolutionary divergence between the two species.
大鼠和兔子的八种核糖体蛋白之间存在差异。这是首次在哺乳动物中精确证明存在这种差异。此外，在这两个物种的肝脏中均观察到一种蛋白水解活性，该活性使蛋白质呈现出被修饰的外观，但在兔网织红细胞中未观察到。这些结果得益于最近开发的一种方法。通过二维聚丙烯酰胺凝胶电泳在四个不同但相关的系统中对核糖体蛋白进行了比较。在四个系统中的每一个中都确定了每种蛋白质的精确位置。发现在一个系统中看似相同的来自不同组织的某些核糖体蛋白在其他系统中是不同的。来自两个不同物种的每个核糖体亚基的蛋白质之间存在显著差异。兔子网织红细胞和肝脏核糖体蛋白之间的差异较小。如果不采取适当措施防止蛋白水解，两个物种的几种肝脏蛋白会改变位置或消失，并且显然会出现新的蛋白质。三种小亚基蛋白和五种大亚基蛋白行为上的差异可归因于这两个物种之间的进化差异。
