Obied Hassan K, Song Yi, Foley Sonia, Loughlin Michael, Rehman Ata-ur, Mailer Rodney, Masud Tariq, Agboola Samson
Graham Centre for Agricultural Innovation, School of Biomedical Sciences, Charles Sturt University , Wagga Wagga, NSW, Australia 2678.
J Agric Food Chem. 2013 Sep 25;61(38):9176-84. doi: 10.1021/jf4026585. Epub 2013 Sep 13.
During the extraction of canola oil, large quantities of meal are produced. Extracting biophenols from Australian canola meal (ACM) adds value to an otherwise low-value agro-industrial byproduct. This study examined the biophenol content and the antioxidant activity of ACM, the impact of extraction conditions, and varietal differences. Sinapine was the principal biophenol in ACM. In crude and hydrolyzed extracts, 31 compounds were identified: 2 dihexosides, 2 organic acids, 4 glucosinolates, 17 sinapic acid derivatives, 2 cyclic spermidine alkaloids, caffeic acid and its dihexoside, kaempferol, and its C-glucoside. ACM showed significant free radical scavenging activity in DPPH(•) and ABTS(•+) assays. Sinapine was the chief contributor to ACM antioxidant activity, whereas kaempferol sinapoyl triglucoside isomer was the most potent antioxidant. Biophenol content ranged between 12.8 and 15.4 mg GAE/g DW. Differences among studied cultivars were generally quantitative. The Tarcoola cultivar showed the highest biophenol content and antioxidant activity.
在菜籽油提取过程中会产生大量粕。从澳大利亚油菜籽粕（ACM）中提取生物酚可提升这种原本价值较低的农业工业副产品的价值。本研究考察了ACM的生物酚含量和抗氧化活性、提取条件的影响以及品种差异。芥子碱是ACM中的主要生物酚。在粗提物和水解提取物中，鉴定出31种化合物：2种二己糖苷、2种有机酸、4种硫代葡萄糖苷、17种芥子酸衍生物、2种环状亚精胺生物碱、咖啡酸及其二己糖苷、山奈酚及其C - 葡萄糖苷。ACM在DPPH(•)和ABTS(•+)测定中表现出显著的自由基清除活性。芥子碱是ACM抗氧化活性的主要贡献者，而山奈酚芥子酰三葡萄糖苷异构体是最有效的抗氧化剂。生物酚含量在12.8至15.4 mg GAE/g干重之间。所研究品种之间的差异一般为数量上的差异。塔尔库拉品种的生物酚含量和抗氧化活性最高。
