Obied H K, Bedgood D R, Prenzler P D, Robards K
E.H. Graham Centre for Agricultural Innovation, School of Science and Technology, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
Food Chem Toxicol. 2007 Jul;45(7):1238-48. doi: 10.1016/j.fct.2007.01.004. Epub 2007 Jan 17.
The biophenolic fraction was characterized in olive mill waste (OMW) obtained as a by-product from processing of Mission and Frantoio olive fruit. OMW produced from the Mission fruit contained higher total phenol content. Individual biophenols with the exception of verbascoside and a hydroxytyrosol-secoiridoid were also present at higher concentrations in the OMW produced from Mission cultivar. Antioxidant activities were measured in aqueous (DPPH) and emulsion (BCBT) systems. The Frantoio extract was more active than the Mission extract in the DPPH assay - EC(50) values were 28.3+/-1.7 ppm and 34.7+/-1.7 ppm, respectively. Activities were reversed in the BCBT, with the Mission extract (EC(50) 60.6+/-2.3 ppm) more potent than the Frantoio extract (EC(50) 79.9+/-2.0 ppm), and this may be related to the more lipophilic nature of the Mission extract. Both extracts showed broad spectrum antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa; whereas individual biophenols (hydroxytyrosol, luteolin, oleuropein) showed more limited activity. Molluscicidal activity was measured against Isidorella newcombi and LD(50) values were 424 ppm and 541 ppm for Mission and Frantoio extracts, respectively. The results suggest that OMW may be utilised as a source of bioactive compounds.
生物酚馏分在作为米申（Mission）和佛朗托伊奥（Frantoio）橄榄果加工副产物获得的橄榄果渣废水（OMW）中得到了表征。米申橄榄果产生的OMW含有更高的总酚含量。除了毛蕊花糖苷和一种羟基酪醇-裂环环烯醚萜苷外，在米申品种产生的OMW中，个别生物酚的浓度也更高。在水相（DPPH）和乳液（BCBT）体系中测定了抗氧化活性。在DPPH测定中，佛朗托伊奥提取物比米申提取物更具活性——EC(50)值分别为28.3±1.7 ppm和34.7±1.7 ppm。在BCBT中活性则相反，米申提取物（EC(50) 60.6±2.3 ppm）比佛朗托伊奥提取物（EC(50) 79.9±2.0 ppm）更有效，这可能与米申提取物更具亲脂性有关。两种提取物对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和铜绿假单胞菌均表现出广谱抗菌活性；而个别生物酚（羟基酪醇、木犀草素、橄榄苦苷）的活性则较为有限。测定了对新氏艾氏螺（Isidorella newcombi）的杀螺活性，米申和佛朗托伊奥提取物的LD(50)值分别为424 ppm和541 ppm。结果表明，OMW可作为生物活性化合物的来源加以利用。
