National Veterinary Research Institute, Department of Virology, Al. Partyzantów 57, 24-100 Puławy, Poland.
Vet Microbiol. 2013 Oct 25;166(3-4):639-44. doi: 10.1016/j.vetmic.2013.07.002. Epub 2013 Jul 10.
The genetic diversity of bovine viral diarrhea virus (BVDV) was determined from 65 animals persistently infected with BVDV and diagnosed between 2004 and 2011 in Poland. The samples originated from 28 herds in 12 provinces, where over 90% of the whole cattle population of Poland is reared. Phylogenetic analysis based on the fragments of two genomic regions of BVDV namely, 5'-untranslated region (5'-UTR) and N(pro) was performed. All the BVDV isolates belonged to BVDV-1 species and were further divided into four subtypes. There were 31 viruses of BVDV-1b subtype (47.6%) present in 12 herds, 24 of BVDV-1d subtype (36.9%) in 9 herds, 8 of BVDV-1f subtype (12.3%) in 5 herds and 2 BVDV-1g subtype (3.0%) in 2 herds. Neither BVDV-1a subtype, nor BVDV-2 species or any atypical bovine pestivirus were found among isolates tested. Despite increasing import of live cattle in the recent years, genetic diversity of Polish BVDV isolates was rather low.
从 2004 年至 2011 年期间，在波兰持续感染牛病毒性腹泻病毒（BVDV）并被诊断出的 65 头动物中，确定了 BVDV 的遗传多样性。这些样本来自波兰 12 个省的 28 个牛群，波兰超过 90%的牛群都饲养在这些地区。对 BVDV 的两个基因组区域即 5'-非翻译区（5'-UTR）和 N(pro)的片段进行了基于遗传进化的分析。所有 BVDV 分离株均属于 BVDV-1 种，并进一步分为四个亚型。在 12 个牛群中存在 31 株 BVDV-1b 亚型病毒（47.6%），9 个牛群中存在 24 株 BVDV-1d 亚型病毒（36.9%），5 个牛群中存在 8 株 BVDV-1f 亚型病毒（12.3%），2 个牛群中存在 2 株 BVDV-1g 亚型病毒（3.0%）。在测试的分离株中，既没有发现 BVDV-1a 亚型，也没有发现 BVDV-2 种或任何非典型牛疱疹病毒。尽管近年来活牛进口量不断增加，但波兰 BVDV 分离株的遗传多样性相当低。
