在线样品预处理阀切换离子色谱法分析阿胶中的六价铬。

Analysis of hexavalent chromium in Colla corii asini with on-line sample pretreatment valve-switching ion chromatography.

机构信息

Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.

出版信息

J Chromatogr A. 2013 Aug 30;1305:171-5. doi: 10.1016/j.chroma.2013.07.027. Epub 2013 Jul 11.

DOI:10.1016/j.chroma.2013.07.027

Abstract

An ion chromatography (IC) system with on-line sample pretreatment using valve-switching technique was developed for the determination of hexavalent chromium (Cr(VI)) in Colla corii asini. Colla corii asini is a complicated sample with organics as main matrix. In this work, a polymer-based reversed-phase column was used as a pretreatment column. Via valve-switching technique, sample solution with target ions were eluted from a collection loop to analytical columns, with matrix eliminated on-line. Under the optimized separation conditions, the method showed good linearity (r=0.9998) in the range of 0.004-1.0mg/L and satisfactory repeatability (RSD<3%, n=6). The limit of detection (LOD) was 1.4μg/L (S/N=3). The average spiked recoveries of Cr(VI) were 93.4-102.0%. The result showed that the on-line sample pretreatment IC system was convenient and practical for the determination of trace Cr(VI) in Colla corii asini samples.

摘要

建立了一种采用阀切换技术的在线样品预处理离子色谱（IC）系统，用于测定阿胶中六价铬（Cr(VI)）。阿胶是一种复杂的样品，主要基质为有机物。在这项工作中，使用聚合物反相柱作为预处理柱。通过阀切换技术，将含有目标离子的样品溶液从收集环中洗脱至分析柱，在线去除基质。在优化的分离条件下，该方法在 0.004-1.0mg/L 范围内具有良好的线性（r=0.9998），重复性良好（RSD<3%，n=6）。检测限（LOD）为 1.4μg/L（S/N=3）。Cr(VI)的平均加标回收率为 93.4-102.0%。结果表明，在线样品预处理 IC 系统方便实用，可用于测定阿胶样品中的痕量 Cr(VI)。