Department of Pharmaceutical Care, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Poland.
J Photochem Photobiol B. 2013 Sep 5;126:42-6. doi: 10.1016/j.jphotobiol.2013.07.001. Epub 2013 Jul 9.
UVA radiation stimulates the production of reactive oxygen species (ROS), which react with lipids, proteins and other intracellular molecules leading to oxidative stress, cellular damage and ultimately cell death. There is, therefore, a growing need for substances exhibiting antioxidant activity, which may support repair mechanisms of the skin. This study evaluates the protective effect of the aqueous Oenothera paradoxa Hudziok defatted seeds extract, rich in polyphenolic compounds, against UVA (25 and 50J/cm(2))-induced changes in normal human dermal fibroblasts (NHDFs). The tested extract (0.1-10μg/ml) has decreased, in a concentration-dependent fashion, the UVA-induced release of lactate dehydrogenase (LDH) into the culture medium, the ROS production (with the use of 2',7'-dichlorodihydrofluorescein diacetate) and lipid peroxidation (utilizing redox reactions with ferrous ions) as compared to the control cells (incubated without the extract). Moreover, the extract increased the number of viable (calcein positive) cells decreasing the number of cells in late apoptosis (annexin V-FITC and propidium iodide positive). Thus our results show that O. paradoxa defatted seeds extract may be beneficial for the prevention of UVA skin damage.
UVA 辐射会刺激活性氧（ROS）的产生，ROS 与脂质、蛋白质和其他细胞内分子发生反应，导致氧化应激、细胞损伤，最终导致细胞死亡。因此，人们越来越需要具有抗氧化活性的物质，这些物质可以支持皮肤的修复机制。本研究评估了富含多酚类化合物的 Oenothera paradoxa Hudziok 去脂种子提取物对正常人类真皮成纤维细胞（NHDFs）的 UVA（25 和 50J/cm(2)）诱导变化的保护作用。测试的提取物（0.1-10μg/ml）以浓度依赖的方式降低了 UVA 诱导的乳酸脱氢酶（LDH）释放到培养基中、ROS 产生（使用 2'，7'-二氯二氢荧光素二乙酸酯）和脂质过氧化（利用亚铁离子的氧化还原反应），与未用提取物孵育的对照细胞相比。此外，提取物增加了活细胞（钙黄绿素阳性）的数量，减少了晚期凋亡细胞的数量（ Annexin V-FITC 和碘化丙啶阳性）。因此，我们的结果表明，O. paradoxa 去脂种子提取物可能有益于预防 UVA 皮肤损伤。
