Department of Microbiology, University of Ibadan, Ibadan, Nigeria,
Folia Microbiol (Praha). 2014 Jan;59(1):69-77. doi: 10.1007/s12223-013-0270-6. Epub 2013 Jul 28.
Animals including food animals play a significant role in the epidemiology of Salmonella enterica. The control requires identification of sources and institution of targeted interventions. This study investigates the diversity of S. enterica serovars, antimicrobial susceptibility, and occurrence of plasmid-mediated quinolone resistance (PMQR) genes in pigs in Ibadan, Nigeria. Pooled fresh pen floor fecal samples of pigs collected from 31 pig farms were cultured; the Salmonella isolates were serotyped and their antimicrobial susceptibility was determined. PMQR genes were screened by polymerase chain reaction. The 229 Salmonella isolates were made of 50 serovars predominated by rare serovars Salmonella Give (n = 36; 15.7 %), Salmonella Brancaster (n = 17; 7.4 %), Salmonella Colindale (n = 15; 6.6 %), Salmonella Elisaberthville (n = 13; 5.7 %), Salmonella Hillingdon (n = 13; 5.7 %), and Salmonella Kingston (n = 13; 5.7 %). The most widely distributed serovars among the farms were Salmonella Give (six farms) and Salmonella Elisaberthville (six farms). Resistance to chloramphenicol, sulfonamides, nalidixic acid, streptomycin, and tetracycline ranged from 11.6 % (n = 26) to 22.8 % (n = 51). Resistance ciprofloxacin and gentamicin was low (n = 2; 0.9 %). Multiply resistant isolates included Salmonella Kentucky, the most resistant serovar. qnrB19 was found in two isolates of Salmonella Corvallis and one isolate of Salmonella Larochelle, respectively, while qnrS1 was found in two isolates of Salmonella Derby. Other PMQR genes were not detected. Pigs constitute an important source of diverse Salmonella serovars in Ibadan. The isolates were more resistant to old antimicrobials with some multiple resistant. Control measures and regulation of antimicrobials are warranted.
动物，包括食用动物，在沙门氏菌属的流行病学中起着重要作用。控制需要识别来源并实施有针对性的干预措施。本研究调查了尼日利亚伊巴丹猪中沙门氏菌血清型的多样性、抗菌药物敏感性以及质粒介导的喹诺酮耐药（PMQR）基因的发生情况。从 31 个养猪场采集的新鲜猪圈粪便样本进行混合培养；分离出的沙门氏菌进行血清分型，并测定其抗菌药物敏感性。通过聚合酶链反应筛选 PMQR 基因。从 229 株沙门氏菌分离株中，发现了 50 种血清型，以罕见血清型沙门氏菌 Give（n=36；15.7%）、沙门氏菌 Brancaster（n=17；7.4%）、沙门氏菌 Colindale（n=15；6.6%）、沙门氏菌 Elisaberthville（n=13；5.7%）、沙门氏菌 Hillingdon（n=13；5.7%）和沙门氏菌 Kingston（n=13；5.7%）为主。在各个农场分布最广的血清型是沙门氏菌 Give（六个农场）和沙门氏菌 Elisaberthville（六个农场）。对氯霉素、磺胺类药物、萘啶酸、链霉素和四环素的耐药率从 11.6%（n=26）到 22.8%（n=51）不等。对环丙沙星和庆大霉素的耐药性较低（n=2；0.9%）。多药耐药株包括耐药性最强的血清型沙门氏菌 Kentucky。在两个沙门氏菌 Corvallis 分离株和一个沙门氏菌 Larochelle 分离株中发现了 qnrB19，而在两个沙门氏菌 Derby 分离株中发现了 qnrS1。其他 PMQR 基因未检出。猪是伊巴丹不同沙门氏菌血清型的重要来源。这些分离株对旧的抗菌药物更具耐药性，其中一些为多重耐药。需要采取控制措施和规范抗菌药物的使用。
