Korea Polar Research Institute, Yeonsu-gu, Incheon, Korea.
PLoS One. 2013 Jul 23;8(7):e68824. doi: 10.1371/journal.pone.0068824. Print 2013.
Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.
下一代测序已成为基因组学研究中应用最广泛的测序技术，但在处理高 GC 含量基因组时存在固有缺陷。最近，单分子实时测序技术（SMRT）被引入作为第三代测序策略来弥补这一缺陷。在这里，我们报告称，SMRT 测序的无偏性和更长的读长通过填补缺口和解决重复问题，显著提高了高 GC 含量基因组的组装质量。
