Raczkowska Katarzyna, Zalewska-Szajda Beata, Chojnowska Sylwia, Kepka Alina, Raczkowski Krzysztof, Waszkiewicz Napoleon, Siedlecka-Czykier Edyta, Dadan Jacek, Snarska Jadwiga, Zwierz Krzysztof, Ładny Jerzy Robert, Szajda Sławomir Dariusz
Wyzsza Szkoła Zawodowa Ochrony Zdrowia Towarzystwa Wiedzy Powszechnej w Łomzy.
Pol Merkur Lekarski. 2013 May;34(203):259-62.
Parenteral nutrition entails numerous metabolic complications resulting from food bypass of the gastrointestinal tract. Up to now have not been established all complications of parenteral nutrition, despite intensive research and clinical observations. Knowledge of the biochemical changes resulting from parenteral nutrition is essential to effective prevention, early detection and effective treatment of the metabolic disorders induced by parenteral nutrition. The aim of the study was to evaluate the catabolism of glycoconjugates of parenterally fed patients, reflected by the activity of N-acetyl-beta-D-hexosaminidase (HEX): HEX A and HEX B isoenzymes in serum and urine.
Samples of blood and urine were collected from 23 patients: before intravenous alimentation, at start, as well as of fifth and tenth day of parenteral nutrition. The activity of HEX A and HEX B in serum and urine was determined by the colorimetric method of Zwierz et al. as modified by Marciniak et al. The activity of urinary HEXA and HEX B has been calculated per 1 mg of creatinine.
The activity of serum HEXA significantly decreased at fifth day, in comparison to the activity before parenteral alimentation, and significantly increased at tenth day of parenteral nutrition. The activity of HEX B in serum increased significantly at fifth and tenth day of the parenteral nutrition.
Parenteral nutrition alter the catabolism of glycoconjugates, reflected by significant changes in serum HEX A and HEX B activities. Urine was the not appropriate material to evaluate the catabolism of glycoconjugates in view of HEX A and HEX B activities.
肠外营养会引发诸多因食物绕过胃肠道而导致的代谢并发症。尽管进行了深入研究和临床观察，但迄今为止，肠外营养的所有并发症尚未完全明确。了解肠外营养引起的生化变化对于有效预防、早期发现和有效治疗肠外营养所致的代谢紊乱至关重要。本研究的目的是评估肠外营养患者糖缀合物的分解代谢情况，以血清和尿液中N - 乙酰 - β - D - 己糖胺酶（HEX）：HEX A和HEX B同工酶的活性为指标。
收集23例患者的血液和尿液样本：静脉营养前、开始时以及肠外营养的第5天和第10天。血清和尿液中HEX A和HEX B的活性采用Zwierz等人经Marciniak等人修改后的比色法测定。尿HEXA和HEX B的活性以每1毫克肌酐计算。
与肠外营养前相比，血清HEXA活性在第5天显著降低，在肠外营养第10天显著升高。血清中HEX B活性在肠外营养第5天和第10天显著升高。
肠外营养改变了糖缀合物的分解代谢，血清HEX A和HEX B活性的显著变化反映了这一点。鉴于HEX A和HEX B的活性，尿液不是评估糖缀合物分解代谢的合适材料。
