Epigenetics Program, Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, 1009C Stellar-Chance Laboratories, 422 Curie Boulevard, Philadelphia, PA 19104, USA.
Epigenomics. 2013 Aug;5(4):439-52. doi: 10.2217/epi.13.37.
Epigenetics is the study of changes in gene expression or cellular phenotype that do not change the DNA sequence. In this review, current methods, both genomic and proteomic, associated with epigenetics research are discussed. Among them, chromatin immunoprecipitation (ChIP) followed by sequencing and other ChIP-based techniques are powerful techniques for genome-wide profiling of DNA-binding proteins, histone post-translational modifications or nucleosome positions. However, mass spectrometry-based proteomics is increasingly being used in functional biological studies and has proved to be an indispensable tool to characterize histone modifications, as well as DNA-protein and protein-protein interactions. With the development of genomic and proteomic approaches, combination of ChIP and mass spectrometry has the potential to expand our knowledge of epigenetics research to a higher level.
表观遗传学是研究基因表达或细胞表型的变化，而这些变化并不改变 DNA 序列。在这篇综述中，讨论了与表观遗传学研究相关的当前的基因组和蛋白质组学方法。其中，染色质免疫沉淀（ChIP）测序和其他基于 ChIP 的技术是用于全基因组分析 DNA 结合蛋白、组蛋白翻译后修饰或核小体位置的强大技术。然而，基于质谱的蛋白质组学在功能生物学研究中越来越多地被应用，并已被证明是一种不可或缺的工具，用于描述组蛋白修饰以及 DNA-蛋白质和蛋白质-蛋白质相互作用。随着基因组和蛋白质组学方法的发展，ChIP 和质谱的结合有可能将我们对表观遗传学研究的认识提高到一个更高的水平。
