Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
J Appl Physiol (1985). 2010 Sep;109(3):927-33. doi: 10.1152/japplphysiol.00007.2010. Epub 2010 Jan 28.
Epigenetics is increasingly being recognized as a central component of physiological processes as diverse as obesity and circadian rhythms. Primarily acting through DNA methylation and histone posttranslational modifications, epigenetic pathways enable both short- and long-term transcriptional activation and silencing, independently of the underlying genetic sequence. To more quantitatively study the molecular basis of epigenetic regulation in physiological processes, the present review informs the latest techniques to identify and compare novel DNA methylation marks and combinatorial histone modifications across different experimental conditions, and to localize both DNA methylation and histone modifications over specific genomic regions.
表观遗传学越来越被认为是肥胖和昼夜节律等多种生理过程的核心组成部分。表观遗传途径主要通过 DNA 甲基化和组蛋白翻译后修饰起作用，能够独立于潜在的遗传序列，实现短期和长期转录激活和沉默。为了更定量地研究生理过程中表观遗传调控的分子基础，本综述介绍了最新技术，用于鉴定和比较不同实验条件下的新型 DNA 甲基化标记和组合组蛋白修饰，并定位特定基因组区域上的 DNA 甲基化和组蛋白修饰。
