David H. Koch Center for Applied Research of Genitourinary Cancers, Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Curr Opin Cell Biol. 2013 Oct;25(5):659-71. doi: 10.1016/j.ceb.2013.07.001. Epub 2013 Jul 26.
Key steps of cancer progression and therapy response depend upon interactions between cancer cells with the reactive tumour microenvironment. Intravital microscopy enables multi-modal and multi-scale monitoring of cancer progression as a dynamic step-wise process within anatomic and functional niches provided by the microenvironment. These niches deliver cell-derived and matrix-derived signals that enable cell subsets or single cancer cells to survive, migrate, grow, undergo dormancy, and escape immune surveillance. Beyond basic research, intravital microscopy has reached preclinical application to identify mechanisms of tumour-stroma interactions and outcome. We here summarise how n-dimensional 'dynamic histopathology' of tumours by intravital microscopy shapes mechanistic insight into cell-cell and cell-tissue interactions that underlie single-cell and collective cancer invasion, metastatic seeding at distant sites, immune evasion, and therapy responses.
癌症进展和治疗反应的关键步骤取决于癌细胞与反应性肿瘤微环境之间的相互作用。活体显微镜能够对癌症进展进行多模式和多尺度的监测，将其视为微环境提供的解剖和功能小生境中的一个动态逐步过程。这些小生境提供了细胞衍生和基质衍生的信号，使细胞亚群或单个癌细胞能够存活、迁移、生长、休眠和逃避免疫监视。除了基础研究外，活体显微镜已经应用于临床前研究，以确定肿瘤-基质相互作用的机制和结果。在这里，我们总结了活体显微镜如何通过多维“动态组织病理学”来塑造对细胞-细胞和细胞-组织相互作用的机制理解，这些相互作用是单细胞和集体癌症侵袭、远处部位转移播种、免疫逃逸和治疗反应的基础。
