Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Int J Mol Sci. 2013 Jul 26;14(8):15578-94. doi: 10.3390/ijms140815578.
The methanolic extract of Flemingia macrophylla roots exhibited significant estrogenic activity in the transgenic plant assay system which was comparable to the activity of soybean extract. Utilizing estrogenic activity-guided fractionation, one new compound, fleminigin, together with 23 known compounds were isolated from F. macrophylla roots' methanolic extract. The structure of the new compound was identified based on intensive spectroscopic analysis and the full spectral data for one of the isolated compounds, flemichin E, was introduced for the first time in the current investigation. The estrogenic and anti-estrogenic activities of the isolated compounds were evaluated revealing that the isolated isoflavonoids may act as partial estrogen agonists, as well as antagonists. Additionally, the anti-inflammatory and the cytotoxic activities of the isolated compounds were studied. These results suggested the potential applications of F. macrophylla extract and its isolated compounds as selective estrogen receptor modulators (SERMs).
大蔓含羞草根的甲醇提取物在转基因植物检测系统中表现出显著的雌激素活性，与大豆提取物的活性相当。利用雌激素活性导向的分离方法，从大蔓含羞草根的甲醇提取物中分离得到一个新化合物 fleminigin 以及 23 个已知化合物。根据综合光谱分析和一个分离化合物的全光谱数据（flemichin E），确定了新化合物的结构。本研究首次介绍了所分离化合物之一的 flemichin E 的全光谱数据。对分离得到的化合物的雌激素和抗雌激素活性进行了评价，结果表明，分离得到的异黄酮可能作为部分雌激素激动剂和拮抗剂。此外，还研究了分离得到的化合物的抗炎和细胞毒性活性。这些结果表明大蔓含羞草提取物及其分离化合物具有作为选择性雌激素受体调节剂（SERMs）的应用潜力。
