Zeiger Errol
Errol Zeiger Consulting, Chapel Hill, NC, USA.
Methods Mol Biol. 2013;1044:3-26. doi: 10.1007/978-1-62703-529-3_1.
Bacterial mutagenicity tests, specifically the Salmonella and E. coli reverse mutation (Ames) test, are widely used and are usually required before a chemical, drug, pesticide, or food additive can be registered for use. The tests are also widely used for environmental monitoring to detect mutagens in air or water. Their use is based on the showing that a positive result in the test was highly predictive for carcinogenesis. This chapter describes the Salmonella and E. coli tests, presents protocols for their use, and addresses data interpretation and reporting.
细菌致突变性试验，特别是沙门氏菌和大肠杆菌回复突变（艾姆斯）试验，应用广泛，通常在化学品、药物、农药或食品添加剂注册使用前是必需的。这些试验也广泛用于环境监测，以检测空气或水中的诱变剂。它们的应用基于这样一个事实，即试验中的阳性结果对致癌作用具有高度预测性。本章描述了沙门氏菌和大肠杆菌试验，介绍了其使用方案，并讨论了数据解释和报告。
