Department of Pathology, Fletcher Allen Health Care/University of Vermont, 111 Colchester Ave, EP1-122, Burlington, VT 05401, USA.
Am J Clin Pathol. 2013 Aug;140(2):143-8. doi: 10.1309/AJCPU0AYZ5SYQIUK.
Traditional microbial identification methods are based on morphology (both micro- and macroscopic) and biochemical tests that require long incubation periods and a good deal of technologist hands-on time. In addition, many of these methods have some degree of subjectivity. In comparison, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identifies microorganisms from colonies grown on solid media within minutes using very few reagents.
A case-based approach was used to review the strengths and weaknesses of MALDI-TOF MS in clinical microbiology laboratories.
MALDI-TOF MS has been proven to be an accurate and reliable method for organism identification including bacteria, yeast, molds, and mycobacteria. It is rapid, with results often 24 hours earlier than traditional methods, and inexpensive. There are no FDA-approved systems available currently. Susceptibility data are still reliant on conventional methods. There are genetically similar organisms that cannot be discriminated reliably with this method.
MALDI-TOF MS is an exciting, innovative method for organism identification in the clinical microbiology laboratory.
传统的微生物鉴定方法基于形态学（微观和宏观）和生化测试，需要长时间的孵育期和大量技术人员的实际操作时间。此外，这些方法中的许多都有一定程度的主观性。相比之下，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）使用很少的试剂，在数分钟内即可从固体培养基上生长的菌落中鉴定微生物。
采用基于案例的方法来回顾 MALDI-TOF MS 在临床微生物学实验室中的优缺点。
MALDI-TOF MS 已被证明是一种准确可靠的方法，可用于鉴定细菌、酵母、霉菌和分枝杆菌等生物体。它快速，结果通常比传统方法早 24 小时，而且价格低廉。目前没有获得 FDA 批准的系统。药敏数据仍然依赖于传统方法。还有一些遗传上相似的生物体无法用这种方法可靠地区分。
MALDI-TOF MS 是临床微生物学实验室中一种令人兴奋的创新的生物体鉴定方法。
