Yang Yongqiang, Liu Ruoyu, Xie Haiqiang, Hui Yanting, Jiao Rengang, Gong Yu, Zhang Yiyu
College of Animal Sciences, Guizhou University, Guiyang 550025, China.
J Nanosci Nanotechnol. 2013 Jul;13(7):4521-38. doi: 10.1166/jnn.2013.7756.
Much tremendous break through have been obtained in recent years for nanopore sequencing to achieve the goal of $1000 genome. As a method of single molecule sequencing, nanopore sequencing can discriminate the individual molecules of the target DNA strand rapidly due to the current blockages by translocating the nucleotides through a nano-scale pore. Both the protein-pores and solid-state nanopore channels which called single nanopore sequencing have been studied widely for the application of nanopore sequencing technology. This review will give a detail representation to protein nanopore and solid-state nanopore sequencing. For protein nanopore sequencing technology, we will introduce different nanopore types, device assembly and some challenges still exist at present. We will focus on more research fields for solid-state nanopore sequencing in terms of materials, device assembly, fabricated methods, translocation process and some specific challenges. The review also covers some of the technical advances in the union nanopore sequencing, which include nanopore sequencing combine with exonuclease, hybridization, synthesis and design polymer.
近年来，纳米孔测序在实现1000美元基因组的目标方面取得了巨大突破。作为一种单分子测序方法，纳米孔测序可以通过使核苷酸穿过纳米级孔来造成电流阻断，从而快速区分目标DNA链的单个分子。被称为单纳米孔测序的蛋白质孔和固态纳米孔通道都已被广泛研究用于纳米孔测序技术的应用。本综述将详细介绍蛋白质纳米孔和固态纳米孔测序。对于蛋白质纳米孔测序技术，我们将介绍不同的纳米孔类型、设备组装以及目前仍然存在的一些挑战。我们将在材料、设备组装、制造方法、转运过程和一些特定挑战方面重点介绍固态纳米孔测序的更多研究领域。该综述还涵盖了联合纳米孔测序的一些技术进展，其中包括纳米孔测序与核酸外切酶、杂交、合成和设计聚合物的结合。
