Zhou You, Ren Meishen, Zhang Pengfei, Jiang Dike, Yao Xueping, Luo Yan, Yang Zexiao, Wang Yin
Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Nanomaterials (Basel). 2022 May 2;12(9):1534. doi: 10.3390/nano12091534.
Foodborne pathogens have become the subject of intense interest because of their high incidence and mortality worldwide. In the past few decades, people have developed many methods to solve this challenge. At present, methods such as traditional microbial culture methods, nucleic acid or protein-based pathogen detection methods, and whole-genome analysis are widely used in the detection of pathogenic microorganisms in food. However, these methods are limited by time-consuming, cumbersome operations or high costs. The development of nanopore sequencing technology offers the possibility to address these shortcomings. Nanopore sequencing, a third-generation technology, has the advantages of simple operation, high sensitivity, real-time sequencing, and low turnaround time. It can be widely used in the rapid detection and serotyping of foodborne pathogens. This review article discusses foodborne diseases, the principle of nanopore sequencing technology, the application of nanopore sequencing technology in foodborne pathogens detection, as well as its development prospects.
食源性病原体因其在全球范围内的高发病率和死亡率而成为人们密切关注的对象。在过去几十年里，人们开发了许多方法来应对这一挑战。目前，传统微生物培养方法、基于核酸或蛋白质的病原体检测方法以及全基因组分析等方法广泛应用于食品中致病微生物的检测。然而，这些方法受到耗时、操作繁琐或成本高昂的限制。纳米孔测序技术的发展为解决这些缺点提供了可能。纳米孔测序作为第三代技术，具有操作简单、灵敏度高、实时测序和周转时间短等优点。它可广泛应用于食源性病原体的快速检测和血清分型。本文综述了食源性疾病、纳米孔测序技术的原理、纳米孔测序技术在食源性病原体检测中的应用及其发展前景。
