[采用高效液相色谱法对山参和园参进行指纹图谱比较]
[Fingerprint comparison of mountain cultivated ginseng and wild ginseng by HPLC].
作者信息
Xu Shi-Yi, Sun Guo-Xiang, Mu Shan-Xue, Sun Qi-Shi
机构信息
Shenyang Pharmaceutical University, Shenyang 110016, China.
出版信息
Zhong Yao Cai. 2013 Feb;36(2):213-6.
OBJECTIVE
To establish the fingerprint of mountain cultivated ginseng by HPLC and compare the fingerprint with that of wild ginseng for the quality control.
METHODS
The analysis was carried out on a ZORBAX Eclipse XDB-C18 column (150 mm x 4.6 mm, 5 microm) with a mobile phase consisting of water-acetonitrile with gradient elution, and the flow rate was 1 mL/min. The column temperature was set at (30 +/- 0.15) degrees C and UV detection wavelength was set at 203 nm.
RESULTS
There were 15 common peaks in the fingerprint of mountain cultivated ginseng. The similarities of 10 batches ginseng were between 0.90 and 1.00.
CONCLUSION
This method has good characteristics and specificity, and could be used for quality control of mountain cultivated ginseng. The type and content of components in wild ginseng were slightly higher than those in mountain cultivation ones.
目的
采用高效液相色谱法建立园参指纹图谱，并与野山参指纹图谱进行比较，以控制其质量。
方法
采用ZORBAX Eclipse XDB-C18色谱柱（150 mm×4.6 mm，5μm），以水-乙腈为流动相进行梯度洗脱，流速为1 mL/min。柱温设定为（30±0.15）℃，紫外检测波长设定为203 nm。
结果
园参指纹图谱共有15个共有峰。10批人参相似度在0.90至1.00之间。
结论
该方法具有良好的专属性和特征性，可用于园参的质量控制。野山参中成分的种类和含量略高于园参。
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