College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi, 712100, China.
New Phytol. 2013 Nov;200(3):834-846. doi: 10.1111/nph.12418. Epub 2013 Jul 31.
Ubiquitin-mediated regulation responds rapidly to specific stimuli; this rapidity is particularly important for defense responses to pathogen attack. Here, we investigated the role of the E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1 (EIRP1) in the defense response of Chinese wild grapevine Vitis pseudoreticulata. The regulatory function of E3 ubiquitin ligase EIRP1 was investigated using molecular, genetic and biochemical approaches. EIRP1 encodes a C3HC4-type Really Interesting New Gene (RING) finger protein that harbors E3 ligase activity. This activity requires the conserved RING domain, and VpWRKY11 also interacts with EIRP1 through the RING domain. VpWRKY11 localizes to the nucleus and activates W-box-dependent transcription in planta. EIRP1 targeted VpWRKY11 in vivo, resulting in VpWRKY11 degradation. The expression of EIRP1 and VpWRKY11 responds rapidly to powdery mildew in Vitis pseudoreticulata grapevine; also, overexpression of EIRP1 in Arabidopsis confers enhanced resistance to the pathogens Golovinomyces cichoracearum and Pseudomonas syringae pv tomato DC3000. Our data suggest that the EIRP1 E3 ligase positively regulates plant disease resistance by mediating proteolysis of the negative regulator VpWRKY11 via degradation by the 26S proteasome.
泛素介导的调节对特定刺激迅速做出反应；这种快速性对于防御病原体攻击特别重要。在这里，我们研究了 Erysiphe necator 诱导的 RING 指蛋白 1（EIRP1）在中华野生葡萄（Vitis pseudoreticulata）防御反应中的作用。使用分子、遗传和生化方法研究了 E3 泛素连接酶 EIRP1 的调节功能。EIRP1 编码 C3HC4 型 Really Interesting New Gene（RING）指蛋白，具有 E3 连接酶活性。这种活性需要保守的 RING 结构域，VpWRKY11 也通过 RING 结构域与 EIRP1 相互作用。VpWRKY11 定位于细胞核内，并在体内激活 W-box 依赖性转录。EIRP1 在体内靶向 VpWRKY11，导致 VpWRKY11 降解。EIRP1 和 VpWRKY11 的表达在中华野生葡萄白粉病中迅速响应；此外，在拟南芥中过表达 EIRP1 赋予对病原体禾谷多黏菌和丁香假单胞菌 pv tomato DC3000 的增强抗性。我们的数据表明，EIRP1 E3 连接酶通过 26S 蛋白酶体介导的负调节因子 VpWRKY11 的降解，正向调节植物的抗病性。
