Department of Veterinary Medicine, Federal University of Lavras, Lavras, MG 37200-000, Brazil.
Institute of Agricultural Sciences, Federal University of Jequitinhonha and Mucuri Valleys, Unaí, MG 38610-000, Brazil.
Nutrients. 2018 Nov 7;10(11):1702. doi: 10.3390/nu10111702.
The authors aimed to evaluate the effects of physical exercise on the metabolism and progression of periodontal disease (PD), induced by ligature in diabetic rats induced by high fat diet and streptozotocin (HFD/STZ). Diabetes Mellitus (DM) was induced by four weeks of a hyperlipidic diet associated with a single low-dose of streptozotocin (35 mg/kg/animal). The exercise groups swam for 60 min/day for eight weeks (five times/week). In the last two weeks of exercise, a ligature was placed around the right and left mandibular first molars. The authors determined alveolar bone loss by morphometry. Blood biochemical profile and serum levels of IL-10 and TNF-α were evaluated by colorimetric and enzyme-linked immunosorbent assays (ELISA), respectively. The diabetic animals subjected to exercise showed decreased alveolar bone loss, lower glycemia, triacylglycerols and glycosylated hemoglobin levels than the controls. Total cholesterol and its fractions (High density lipoprotein-HDL-c, Low density lipoprotein-LDL-c and Very low density lipoprotein-VLDL-c) remained similar among the groups. Animals with PD showed higher levels of TNF-α and lower levels of IL-10, when compared to animals without PD. In diabetic animals with PD, physical exercise decreased TNF-α levels and increased IL-10 levels as well as the IL10/TNF-α ratio. In conclusion, eight weeks of physical exercise improved glycemic control and systemic inflammatory profile, and attenuated alveolar bone loss in rats with DM and PD.
作者旨在评估运动对高脂饮食和链脲佐菌素（HFD/STZ）诱导的糖尿病大鼠牙周病（PD）代谢和进展的影响。糖尿病（DM）通过四周高脂饮食和单次低剂量链脲佐菌素（35mg/kg/动物）诱导。运动组每天游泳 60 分钟，共 8 周（每周 5 次）。在运动的最后两周，在下颌左右第一磨牙周围放置结扎线。作者通过形态计量学来确定牙槽骨丢失。通过比色法和酶联免疫吸附测定（ELISA）分别评估血液生化谱和血清中白细胞介素 10（IL-10）和肿瘤坏死因子-α（TNF-α）水平。与对照组相比，运动的糖尿病动物表现出较低的牙槽骨丢失、较低的血糖、三酰甘油和糖化血红蛋白水平。各组总胆固醇及其分数（高密度脂蛋白胆固醇-HDL-c、低密度脂蛋白胆固醇-LDL-c 和极低密度脂蛋白胆固醇-VLDL-c）相似。与无 PD 的动物相比，PD 动物的 TNF-α水平更高，IL-10 水平更低。在患有 PD 的糖尿病动物中，运动降低了 TNF-α水平，增加了 IL-10 水平以及 IL10/TNF-α 比值。总之，8 周的运动改善了血糖控制和全身炎症谱，并减轻了糖尿病和 PD 大鼠的牙槽骨丢失。
