Jamdagni Pragati, Nehra Kiran, Rana J S, Temple Louise M
Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal - 131039, Sonipat, Haryana, India.
School of Integrated Sciences, James Madison University, Harrisonburg, Virginia, USA.
Data Brief. 2023 Mar 31;48:109104. doi: 10.1016/j.dib.2023.109104. eCollection 2023 Jun.
Two novel mycobacteriophages (Prann and LeoAvram) belonging to the family Siphoviridae were isolated from soil samples of Northern India. Genomic DNA of both the phages was extracted, and further sequenced using Illumina technology. Complete genome annotation of both the isolates was performed using DNA Master. Prann and LeoAvram had linear genomes of 68398bp and 47079bp, respectively, with G+C contents of 60-70%. A total of 99 and 75 ORFs were predicted in Prann and LeoAvram, respectively. Based on sequence similarity to known phage proteins, functions were assigned to 44 and 53 genes, respectively. These proteins could be classified into five major groups, ., phage structural proteins, proteins for recombination, lytic enzymes, proteins involved in DNA / RNA metabolism, and in regulation. was used in this work as a safe surrogate for , the causative agent for tuberculosis, a major infectious disease worldwide with developing antibiotic resistance. This is the first report of phages from Northern India.
从印度北部的土壤样本中分离出两种属于长尾噬菌体科的新型分枝杆菌噬菌体（Prann和LeoAvram）。提取了这两种噬菌体的基因组DNA，并使用Illumina技术进一步测序。使用DNA Master对这两种分离株进行了完整的基因组注释。Prann和LeoAvram的线性基因组分别为68398bp和47079bp，G+C含量为60%-70%。在Prann和LeoAvram中分别预测到99个和75个开放阅读框（ORF）。基于与已知噬菌体蛋白的序列相似性，分别为44个和53个基因赋予了功能。这些蛋白质可分为五大类，即噬菌体结构蛋白、重组蛋白、裂解酶、参与DNA/RNA代谢的蛋白以及调节蛋白。在这项工作中，使用[具体内容缺失]作为[具体内容缺失]的安全替代物，[具体内容缺失]是结核病的病原体，结核病是一种在全球范围内具有抗生素耐药性的主要传染病。这是来自印度北部[具体内容缺失]噬菌体的首次报道。
