College of Engineering, Peking University, Beijing, 100871, China.
School of Biological Science and Technology, University of Jinan, Shandong, 250022, China.
Arch Virol. 2021 Feb;166(2):601-606. doi: 10.1007/s00705-020-04912-z. Epub 2021 Jan 3.
Bacteriophage 8P was isolated with a Pseudomonas stutzeri strain isolated from an oil reservoir as its host bacterium. The phage genome comprises 63,753 base pairs with a G+C content of 64.35. The phage encodes 63 predicted proteins, and 27 of them were functionally assigned. No tRNA genes were found. Comparative genomics analysis showed that 8P displayed some relatedness to F116-like phages (78% identity, 20% query coverage). The genome has very low sequence similarity to the other phage genomes in the GenBank database and Viral Sequence Database. Based on whole-genome analysis and transmission electron microscopy imaging, 8P is proposed to be a member of a new species in the genus Hollowayvirus, family Podoviridae.
噬菌体 8P 是从一个油藏中分离出来的假单胞菌菌株作为宿主菌而分离出来的。噬菌体基因组由 63753 个碱基对组成，G+C 含量为 64.35%。噬菌体编码 63 个预测蛋白，其中 27 个具有功能。未发现 tRNA 基因。比较基因组学分析表明，8P 与 F116 样噬菌体具有一定的亲缘关系（78%的同一性，20%的查询覆盖率）。基因组与 GenBank 数据库和病毒序列数据库中的其他噬菌体基因组的序列相似度非常低。基于全基因组分析和透射电子显微镜成像，建议 8P 是 Hollowayvirus 属、细小病毒科的一个新种成员。
