Kim Hyun-Sook, Bang John J, Lee Sang-Seob
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Department of Environmental, Earth and Geospatial Science, North Carolina Central University, Fayettevile St., Durham, NC, 27707, USA.
Curr Microbiol. 2017 Nov;74(11):1247-1252. doi: 10.1007/s00284-017-1280-4. Epub 2017 Sep 14.
A Gram-stain-negative bacterial strain designated Con4 was isolated from activated sludge in a seawater treatment system. The strain was rod-shaped, motile, aerobic, and formed yellow-colored colonies on agar medium. Cells contained carotenoid pigments, but flexirubin-type pigments were absent. Based on 16S rRNA gene sequence analysis, the strain was assigned to the genus Gelidibacter. Optimum growth occurred at 20 °C, pH 7.0, and 1-2% (w/v) NaCl. Prominent fatty acid types were iso-C, anteiso-C, and iso-C 3OH. Diphosphatidylglycerol and phosphatidylethanolamine were present as the major polar lipids. MK-6 was present as major menaquinone. Strain Con4 showed the highest sequence similarity with Gelidibacter mesophilus KCTC 12106 (96.5%), Gelidibacter gilvus IC158 (96.4%), and Gelidibacter algens ACAM 536 (95.8%). The G+C mol% contents of the strain Con4 were 37.7%. Distinct morphological, physiological, and genotypic differences from the previously described taxa support the classification of strain Con4 as a representative of a novel species in the genus Gelidibacter, for which the name Gelidibacter flavus sp. nov. is proposed. The type strain is Con4 (=KEMB 41-198 = JCM 31135).
从海水处理系统的活性污泥中分离出一株革兰氏阴性细菌菌株，命名为Con4。该菌株呈杆状，具运动性，好氧，在琼脂培养基上形成黄色菌落。细胞含有类胡萝卜素色素，但不存在柔韧菌素型色素。基于16S rRNA基因序列分析，该菌株被归入嗜冷栖热袍菌属。最适生长温度为20℃，pH值为7.0，NaCl浓度为1-2%（w/v）。主要脂肪酸类型为异-C、anteiso-C和异-C 3OH。二磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂质存在。MK-6作为主要甲基萘醌存在。菌株Con4与嗜温嗜冷栖热袍菌KCTC 12106（96.5%）、吉尔嗜冷栖热袍菌IC158（96.4%）和产藻嗜冷栖热袍菌ACAM 536（95.8%）的序列相似性最高。菌株Con4的G+C mol%含量为37.7%。与先前描述的分类单元在形态、生理和基因型上的明显差异支持将菌株Con4分类为嗜冷栖热袍菌属一个新物种的代表，为此提出新名称嗜黄嗜冷栖热袍菌。模式菌株为Con4（=KEMB 41-198 = JCM 31135）。
