Srinivasan Sathiyaraj, Lee Jae-Jin, Lee Sang-Seob, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
Curr Microbiol. 2014 Sep;69(3):263-9. doi: 10.1007/s00284-014-0580-1. Epub 2014 Apr 20.
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated as SWU8(T), was isolated from a mountain soil collected in Seoul Women's University campus at South Korea. Phylogenic analysis, using 16S rRNA gene sequence of the new isolate, showed that strain SWU8(T) belongs to the genus Pontibacter. The highest sequence similarities were 96.2 % with Pontibacter saemangeumensis GCM0142(T), 95.5 % with Pontibacter toksunensis ZLD-7(T), 95.3 % with Pontibacter roseus DSM 17521(T), and 95.1 % with Pontibacter odishensis JC130(T). Chemotaxonomic data showed that the most abundant fatty acids were summed feature 4 (comprising iso-C(17:1) I/anteiso-C(17:1) B; 26.9 %), iso-C(15:0) (25.6 %), and iso-C(17:0) 3OH (10.6 %), and major polar lipid was phosphatidylethanolamine. The DNA G+C content of strain SWU8(T) was 48.5 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain SWU8(T) presents a novel species of the genus Pontibacter, for which the name Pontibacter humi sp. nov. is proposed. The type strain is SWU8(T) (=KEMC 9004-131(T) = JCM 19178(T)).
从韩国首尔女子大学校园采集的山地土壤中分离出一株革兰氏阴性、需氧、无运动性、杆状的细菌菌株，命名为SWU8(T)。利用新分离菌株的16S rRNA基因序列进行系统发育分析，结果表明菌株SWU8(T)属于Pontibacter属。与盐满湖Pontibacter菌GCM0142(T)的序列相似性最高，为96.2%；与德孙Pontibacter菌ZLD-7(T)的相似性为95.5%；与玫瑰Pontibacter菌DSM 17521(T)的相似性为95.3%；与奥迪申斯Pontibacter菌JC130(T)的相似性为95.1%。化学分类学数据显示，最丰富的脂肪酸是特征性总和4（包括异-C(17:1)I/anteiso-C(17:1)B；26.9%）、异-C(15:0)（25.6%）和异-C(17:0) 3OH（10.6%），主要极性脂为磷脂酰乙醇胺。菌株SWU8(T)的DNA G+C含量为48.5 mol%。综合表型、系统发育和化学分类学数据表明，菌株SWU8(T)代表Pontibacter属的一个新物种，为此提出Pontibacter humi sp. nov.的名称。模式菌株为SWU8(T)（=KEMC 9004-131(T)=JCM 19178(T)）。
