Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China.
State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4574-4579. doi: 10.1099/ijs.0.052274-0. Epub 2013 Aug 1.
A Gram-stain-negative, short-rod-shaped bacterium, designated 22DY03(T), was isolated from a sediment sample collected from the East Pacific Rise. The isolate required NaCl and grew best with 3-7 % (w/v) sea salts at temperature of between 30 and 35 °C at pH 7.0. It formed non-pigmented colonies and produced exopolysaccharide, but did not produce bacteriochlorophyll a. Strain 22DY03(T) was positive for hydrolysis of aesculin and Tween 20 and negative for hydrolysis of casein, DNA, gelatin, starch and Tween 40, 60 and 80. The major respiratory quinone was ubiquinone-10. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, two unidentified phospholipids and four unidentified polar lipids. The major fatty acids were C19 : 0 cyclo ω8c, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The genomic DNA G+C content was 64.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain 22DY03(T) should be assigned to the genus Roseivivax. The 16S rRNA gene sequence similarities between the isolate and the type strains of species of the genus Roseivivax were in the range of 94.1-95.8 %. On the basis of phenotypic and genotypic data, it is concluded that strain 22DY03(T) represents a novel species of the genus Roseivivax, for which the name Roseivivax pacificus sp. nov. (type strain 22DY03(T) = CGMCC 1.12410(T) = JCM 18866(T)) is proposed.
一株革兰氏阴性、短杆状细菌，被命名为 22DY03(T)，从东太平洋海隆采集的沉积物样本中分离得到。该分离株需要氯化钠，在 30-35°C 的温度下，在 3-7%（w/v）海盐中生长最好，在 pH7.0 时。它形成非色素菌落并产生胞外多糖，但不产生细菌叶绿素 a。22DY03(T)菌株阳性水解表儿茶素和吐温 20，阴性水解酪蛋白、DNA、明胶、淀粉和吐温 40、60 和 80。主要呼吸醌为泛醌-10。极性脂谱由磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、双磷脂酰甘油、两种未鉴定的磷脂和四种未鉴定的极性脂组成。主要脂肪酸为 C19:0 环 ω8c、C18:1ω7c 和 11-甲基 C18:1ω7c。基因组 DNA G+C 含量为 64.6mol%。基于 16S rRNA 基因序列的系统发育分析表明，22DY03(T)菌株应归属于玫瑰单胞菌属。该分离株与玫瑰单胞菌属种的模式菌株的 16S rRNA 基因序列相似性在 94.1-95.8%之间。基于表型和基因型数据，结论是 22DY03(T)菌株代表了玫瑰单胞菌属的一个新种，因此建议将其命名为太平洋玫瑰单胞菌新种（模式菌株 22DY03(T) = CGMCC 1.12410(T) = JCM 18866(T)）。
